Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

M. I. Mhlongo; F. Tugizimana; L. A. Piater; P. A. Steenkamp; N. E. Madala; I. A. Dubery

doi:10.1016/j.bbrc.2016.12.063

Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

M. I. Mhlongo, F. Tugizimana, L. A. Piater, P. A. Steenkamp, N. E. Madala, I. A. Dubery

Biochemistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells.

Original language	English
Pages (from-to)	1498-1503
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	482
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2016.12.063
Publication status	Published - 22 Jan 2017

Keywords

Azelaic acid
Innate immunity
Lipopolysaccharide
Metabolomics
Phytohormones
Salicylic acid

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2016.12.063

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@article{48213cc90859420bb3345ead6254f3e2,

title = "Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells",

abstract = "To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells.",

keywords = "Azelaic acid, Innate immunity, Lipopolysaccharide, Metabolomics, Phytohormones, Salicylic acid",

author = "Mhlongo, {M. I.} and F. Tugizimana and Piater, {L. A.} and Steenkamp, {P. A.} and Madala, {N. E.} and Dubery, {I. A.}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2017",

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doi = "10.1016/j.bbrc.2016.12.063",

language = "English",

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T1 - Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

AU - Mhlongo, M. I.

AU - Tugizimana, F.

AU - Piater, L. A.

AU - Steenkamp, P. A.

AU - Madala, N. E.

AU - Dubery, I. A.

PY - 2017/1/22

Y1 - 2017/1/22

N2 - To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells.

AB - To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells.

KW - Azelaic acid

KW - Innate immunity

KW - Lipopolysaccharide

KW - Metabolomics

KW - Phytohormones

KW - Salicylic acid

UR - http://www.scopus.com/inward/record.url?scp=85008423049&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2016.12.063

DO - 10.1016/j.bbrc.2016.12.063

M3 - Article

C2 - 27956183

AN - SCOPUS:85008423049

SN - 0006-291X

VL - 482

SP - 1498

EP - 1503

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

Abstract

Keywords

ASJC Scopus subject areas

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