Uncertainty from sampling in measurements of aflatoxins in animal feedingstuffs: Application of the Eurachem/CITAC guidelines

Elisabeth Viktoria Reiter, Mike Francis Dutton, Ali Agus, Erik Nordkvist, Mulunda Feza Mwanza, Patrick Berka Njobeh, Deni Prawano, Per Häggblom, Ebrahim Razzazi-Fazeli, Jürgen Zentek, Mats Gunnar Andersson

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The duplicate method for estimating uncertainty from measurement including sampling is presented in the Eurachem/CITAC guide. The applicability of this method as a tool for verifying sampling plans for mycotoxins was assessed in three case studies with aflatoxin B1 in animal feedingstuffs. Aspects considered included strategies for obtaining samples from contaminated lots, assumptions about distributions, approaches for statistical analysis, log 10-transformation of test data and applicability of uncertainty estimates. The results showed that when duplicate aggregate samples are formed by interpenetrating sampling, repeated measurements from a lot can be assumed to approximately follow a normal or lognormal distribution. Due to the large variation in toxin concentration between sampling targets and sometimes very large uncertainty arising from sampling and sample preparation (Urel ≥ 50%), estimation of uncertainty from log10-transformed data was found to be a more generally applicable approach than application of robust ANOVA.

Original languageEnglish
Pages (from-to)4059-4069
Number of pages11
JournalThe Analyst
Volume136
Issue number19
DOIs
Publication statusPublished - 7 Oct 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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