Towards reliable quantification of hydroxyl radicals in the Fenton reaction using chemical probes

C. Burgos Castillo Rutely, Jean M. Fontmorin, Z. Tang Walter, Dominguez Benetton Xochitl, Sillanpää Mika

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Quantification of hydroxyl radical concentration using two chemical probes was assessed through the Fenton reaction. The probes were 1,2-benzopyrone (coumarin) for fluorescence and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) for electron spin resonance (ESR). The corresponding hydroxylated species, namely 7-hydroxycoumarin (7HC) and 2-hydroxy-5,5-dimethyl-1-pyrroline-N-oxide (DMPO-OH adduct), were monitored by fluorescence and ESR-spin trapping techniques, respectively. The experiments were designed according to the theoretical conditions determined for stable fluorescence and EPR signals. The results demonstrate that: the optimal [chemical probe]:[H2O2] ratio predicted by a simplified quasi-steady-state model was in good agreement with the optimal [chemical probe]:[H2O2] ratio observed experimentally for [H2O2]:[Fe2+] = 10, and the proper adjustment of the [chemical probe]:[H2O2] ratio at a given concentration of the Fenton's reagent improves the detected amount of hydroxyl radicals. Finally, using DMPO required a higher concentration compared to coumarin to yield the same amount of OH detected but resulted in a more reliable probe for detecting OH under the consideration of this study.

Original languageEnglish
Pages (from-to)5321-5330
Number of pages10
JournalRSC Advances
Volume8
Issue number10
DOIs
Publication statusPublished - 2018
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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