Abstract
Biocalorimetric experiments were performed to investigate the metabolic thermal responses of the halotolerant species Staphylococcus lentus in glucose limited mineral salt medium. Growth factors were optimized in both shaker flask and calorimetric experiments. A limiting value of 5 g/L glucose was found to be the optimum for S. lentus growth. The heat flux profiles, OUR, biomass growth, and substrate depletion profiles were compared to deduce the metabolic activity of S. lentus. Shifts in heat flux due to the shifts in substrate uptake and three distinct phases of growth are noticeable in heat profile curves. Respirogram (OUR) and heat profiles are seen to follow the biomass growth curve. Oxycalorific coefficient is validated with the theoretical studies and those noticed in published literature.
| Original language | English |
|---|---|
| Pages (from-to) | 149-155 |
| Number of pages | 7 |
| Journal | Journal of Thermal Analysis and Calorimetry |
| Volume | 104 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 2011 |
| Externally published | Yes |
Keywords
- Bioreaction calorimeter
- Heat yield
- Metabolic activity
- Monod kinetics
- Oxycalorific coefficient
- Staphylococcus lentus
ASJC Scopus subject areas
- Condensed Matter Physics
- Physical and Theoretical Chemistry