The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

Daniela Santos Masson-Meyers, Violet Vakunseh Bumah, Gabriel Biener, Valerica Raicu, Chukuka Samuel Enwemeka

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

It has long been argued that light from a laser diode is superior to light from a light-emitting diode (LED) in terms of its effect on biological tissues. In order to shed light on this ongoing debate, we compared the antimicrobial effect of light emitted from a 405-nm LED with that of a 405-nm laser on methicillin-resistant Staphylococcus aureus (MRSA) at comparable fluences. We cultured 5 × 106 CFU/ml MRSA on tryptic soy agar and then irradiated culture plates once, twice, or thrice with either LED or laser light using 40, 54, 81, or 121 J/cm2 fluence at 15-, 30-, or 240-min time interval between irradiation. Cultures were incubated immediately after irradiation at 37 °C for 24 h before imaging and counting remnant bacterial colonies. Regardless of the device used, LED or laser, irradiation at each fluence resulted in statistically significant bacterial growth suppression compared to non-irradiated controls (p < 0.0001). The antimicrobial effect of both light sources, LED and laser, was not statistically different at each fluence in 35 of the 36 experimental trials. Bacterial growth suppression achieved with either source of light increased with repeated irradiation, particularly at the 15- or 30-min treatment time interval. Thus, we conclude that the antimicrobial effect of 405-nm laser and 405-nm LED on MRSA is similar; neither has a superior antimicrobial effect when compared to the other.

Original languageEnglish
Pages (from-to)2265-2271
Number of pages7
JournalLasers in Medical Science
Volume30
Issue number9
DOIs
Publication statusPublished - 1 Dec 2015
Externally publishedYes

Keywords

  • Antimicrobial therapy
  • Blue light
  • LED versus laser
  • Low-level light therapy
  • Methicillin-resistant Staphylococcus aureus
  • Phototherapy

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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