The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)

I. M. Tynga; N. N. Houreld; H. Abrahamse

doi:10.1016/j.jphotobiol.2012.11.009

The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)

I. M. Tynga, N. N. Houreld, H. Abrahamse

Laser Research Centre

University of Johannesburg

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The development of curative techniques which are selective for neoplasms is one of the main focal areas in cancer research. The mechanism of cell damage due to Zinc phthalocyanine (ZnPcS_mix)-mediated photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) was assessed by inverted light microscopy for morphology, the Trypan blue exclusion assay and adenosine triphosphate (ATP) luminescence assay for cell viability, alamarBlue for proliferation, Lactate Dehydrogenase (LHD) membrane integrity for cytotoxicity and fluorescent microscopy for ZnPcS_mix localization. Fluorescent microscopy revealed that ZnPcS_mix was localized in both mitochondria and lysosomes, and PDT treated cells showed damaging structural changes and decreased cell viability and proliferation. The light-dependent ZnPcS _mix displayed appreciable photosensitivity and the intensity of damage was directly related to its concentration.

Original language	English
Pages (from-to)	171-176
Number of pages	6
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	120
DOIs	https://doi.org/10.1016/j.jphotobiol.2012.11.009
Publication status	Published - 5 Mar 2013

Keywords

Breast cancer
Photodynamic therapy
Zinc phthalocyanine

ASJC Scopus subject areas

Radiation
Radiological and Ultrasound Technology
Biophysics
Radiology, Nuclear Medicine and Imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jphotobiol.2012.11.009

Cite this

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abstract = "The development of curative techniques which are selective for neoplasms is one of the main focal areas in cancer research. The mechanism of cell damage due to Zinc phthalocyanine (ZnPcSmix)-mediated photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) was assessed by inverted light microscopy for morphology, the Trypan blue exclusion assay and adenosine triphosphate (ATP) luminescence assay for cell viability, alamarBlue for proliferation, Lactate Dehydrogenase (LHD) membrane integrity for cytotoxicity and fluorescent microscopy for ZnPcSmix localization. Fluorescent microscopy revealed that ZnPcSmix was localized in both mitochondria and lysosomes, and PDT treated cells showed damaging structural changes and decreased cell viability and proliferation. The light-dependent ZnPcS mix displayed appreciable photosensitivity and the intensity of damage was directly related to its concentration.",

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AU - Abrahamse, H.

PY - 2013/3/5

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AB - The development of curative techniques which are selective for neoplasms is one of the main focal areas in cancer research. The mechanism of cell damage due to Zinc phthalocyanine (ZnPcSmix)-mediated photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) was assessed by inverted light microscopy for morphology, the Trypan blue exclusion assay and adenosine triphosphate (ATP) luminescence assay for cell viability, alamarBlue for proliferation, Lactate Dehydrogenase (LHD) membrane integrity for cytotoxicity and fluorescent microscopy for ZnPcSmix localization. Fluorescent microscopy revealed that ZnPcSmix was localized in both mitochondria and lysosomes, and PDT treated cells showed damaging structural changes and decreased cell viability and proliferation. The light-dependent ZnPcS mix displayed appreciable photosensitivity and the intensity of damage was directly related to its concentration.

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KW - Photodynamic therapy

KW - Zinc phthalocyanine

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The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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