The induction of cell death by phosphine silver(I) thiocyanate complexes in SNO-esophageal cancer cells

Zelinda Human; Appollinaire Munyaneza; Bernard Omondi; Natasha M. Sanabria; Reinout Meijboom; Marianne J. Cronjé

doi:10.1007/s10534-014-9817-5

The induction of cell death by phosphine silver(I) thiocyanate complexes in SNO-esophageal cancer cells

Zelinda Human, Appollinaire Munyaneza, Bernard Omondi, Natasha M. Sanabria, Reinout Meijboom, Marianne J. Cronjé

Chemical Sciences

University of Johannesburg

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Esophageal cancer is one of the least studied cancers and is found to be prominent in black South African males. It is mainly diagnosed in the late stages, and patients tend to have a low 5-year survival rate of only 10 %. Silver is generally used as an antimicrobial agent, with limited reports on anticancer studies. In this study, dimeric silver(I) thiocyanate complexes were used containing a variation of 4-substitued triphenylphosphines, including [AgSCN(PPh₃)₂]₂ (1), [AgSCN {P(4-MeC₆H₄)₃}₂]₂ (2), [AgSCN{P(4-FC₆H₄)₃}₂]₂ (3) and [AgSCN{P(4-ClC₆H₄)₃}₂]₂ (4). All four complexes, with their respective phosphine ligands, PPh₃ (L1) , P(4-MeC₆H₄)₃ (L2), P(4-FC₆H₄)₃ (L3) and P(4-ClC₆H₄)₃(L4), were subjected to in vitro toxicity studies in SNO-esophageal cancer cells, using an alamarBlue® assay. Morphological changes, including blebbing and apoptotic body formation, were observed. Phosphatidylserine externalization, a marker of apoptosis, was quantified by flow cytometry. The phosphine ligands L1-L4, on their own, had minimal effect on the malignant while complexes 1-4 resulted in significant cell death. A 10x decreased concentration of these complexes had similar effects than cisplatin, used as the positive control. These complexes show promise as anticancer agents.

Original language	English
Pages (from-to)	219-228
Number of pages	10
Journal	BioMetals
Volume	28
Issue number	1
DOIs	https://doi.org/10.1007/s10534-014-9817-5
Publication status	Published - Feb 2015

Keywords

Anticancer
Apoptosis
Flow cytometry
Phosphines
SNO-esophageal cancer
Silver(I) thiocyanate complexes

ASJC Scopus subject areas

Biomaterials
General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences
Metals and Alloys

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10534-014-9817-5

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title = "The induction of cell death by phosphine silver(I) thiocyanate complexes in SNO-esophageal cancer cells",

abstract = "Esophageal cancer is one of the least studied cancers and is found to be prominent in black South African males. It is mainly diagnosed in the late stages, and patients tend to have a low 5-year survival rate of only 10 %. Silver is generally used as an antimicrobial agent, with limited reports on anticancer studies. In this study, dimeric silver(I) thiocyanate complexes were used containing a variation of 4-substitued triphenylphosphines, including [AgSCN(PPh3)2]2 (1), [AgSCN {P(4-MeC6H4)3}2]2 (2), [AgSCN{P(4-FC6H4)3}2]2 (3) and [AgSCN{P(4-ClC6H4)3}2]2 (4). All four complexes, with their respective phosphine ligands, PPh3 (L1) , P(4-MeC6H4)3 (L2), P(4-FC6H4)3 (L3) and P(4-ClC6H4)3(L4), were subjected to in vitro toxicity studies in SNO-esophageal cancer cells, using an alamarBlue{\textregistered} assay. Morphological changes, including blebbing and apoptotic body formation, were observed. Phosphatidylserine externalization, a marker of apoptosis, was quantified by flow cytometry. The phosphine ligands L1-L4, on their own, had minimal effect on the malignant while complexes 1-4 resulted in significant cell death. A 10x decreased concentration of these complexes had similar effects than cisplatin, used as the positive control. These complexes show promise as anticancer agents.",

keywords = "Anticancer, Apoptosis, Flow cytometry, Phosphines, SNO-esophageal cancer, Silver(I) thiocyanate complexes",

author = "Zelinda Human and Appollinaire Munyaneza and Bernard Omondi and Sanabria, {Natasha M.} and Reinout Meijboom and Cronj{\'e}, {Marianne J.}",

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doi = "10.1007/s10534-014-9817-5",

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AU - Human, Zelinda

AU - Munyaneza, Appollinaire

AU - Omondi, Bernard

AU - Sanabria, Natasha M.

AU - Meijboom, Reinout

AU - Cronjé, Marianne J.

N1 - Publisher Copyright: © Springer Science+Business Media 2014.

PY - 2015/2

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N2 - Esophageal cancer is one of the least studied cancers and is found to be prominent in black South African males. It is mainly diagnosed in the late stages, and patients tend to have a low 5-year survival rate of only 10 %. Silver is generally used as an antimicrobial agent, with limited reports on anticancer studies. In this study, dimeric silver(I) thiocyanate complexes were used containing a variation of 4-substitued triphenylphosphines, including [AgSCN(PPh3)2]2 (1), [AgSCN {P(4-MeC6H4)3}2]2 (2), [AgSCN{P(4-FC6H4)3}2]2 (3) and [AgSCN{P(4-ClC6H4)3}2]2 (4). All four complexes, with their respective phosphine ligands, PPh3 (L1) , P(4-MeC6H4)3 (L2), P(4-FC6H4)3 (L3) and P(4-ClC6H4)3(L4), were subjected to in vitro toxicity studies in SNO-esophageal cancer cells, using an alamarBlue® assay. Morphological changes, including blebbing and apoptotic body formation, were observed. Phosphatidylserine externalization, a marker of apoptosis, was quantified by flow cytometry. The phosphine ligands L1-L4, on their own, had minimal effect on the malignant while complexes 1-4 resulted in significant cell death. A 10x decreased concentration of these complexes had similar effects than cisplatin, used as the positive control. These complexes show promise as anticancer agents.

AB - Esophageal cancer is one of the least studied cancers and is found to be prominent in black South African males. It is mainly diagnosed in the late stages, and patients tend to have a low 5-year survival rate of only 10 %. Silver is generally used as an antimicrobial agent, with limited reports on anticancer studies. In this study, dimeric silver(I) thiocyanate complexes were used containing a variation of 4-substitued triphenylphosphines, including [AgSCN(PPh3)2]2 (1), [AgSCN {P(4-MeC6H4)3}2]2 (2), [AgSCN{P(4-FC6H4)3}2]2 (3) and [AgSCN{P(4-ClC6H4)3}2]2 (4). All four complexes, with their respective phosphine ligands, PPh3 (L1) , P(4-MeC6H4)3 (L2), P(4-FC6H4)3 (L3) and P(4-ClC6H4)3(L4), were subjected to in vitro toxicity studies in SNO-esophageal cancer cells, using an alamarBlue® assay. Morphological changes, including blebbing and apoptotic body formation, were observed. Phosphatidylserine externalization, a marker of apoptosis, was quantified by flow cytometry. The phosphine ligands L1-L4, on their own, had minimal effect on the malignant while complexes 1-4 resulted in significant cell death. A 10x decreased concentration of these complexes had similar effects than cisplatin, used as the positive control. These complexes show promise as anticancer agents.

KW - Anticancer

KW - Apoptosis

KW - Flow cytometry

KW - Phosphines

KW - SNO-esophageal cancer

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M3 - Article

C2 - 25547071

AN - SCOPUS:84925503807

SN - 0966-0844

VL - 28

SP - 219

EP - 228

JO - BioMetals

JF - BioMetals

IS - 1

ER -

The induction of cell death by phosphine silver(I) thiocyanate complexes in SNO-esophageal cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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