The genetic organization of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni

Burtram Clinton Fielding; Sehaam Khan; Weizhou Wang; Courtney Kruger; Rayaana Abrahams; Sean Davison

doi:10.1023/A:1020118007875

The genetic organization of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni

Burtram Clinton Fielding, Sehaam Khan, Weizhou Wang, Courtney Kruger, Rayaana Abrahams, Sean Davison

University of the Western Cape

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In order to investigate the genomic organization of the Trichoplusia ni Single Capsid Nucleopolyhedrovirus (TnSNPV), a 2,966 basepairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading frames (ORFs) homologous to baculovirus genes, including p26, fibrillin (p10), AcMNPV ORF-29, late expression factor 6 (lef-6) and the C-terminal portion of p74, on either strand of DNA. Predicted amino acid sequences for the ORFs were compared and identity values of between 12% and 54% were observed. TnSNPV has previously been tentatively identified as a member of the Group II NPVs. Clustering and arrangement of the TnSNPV genes were similar to the clustering reported for SeMNPV, confirming TnSNPV as a Group II NPV.

Original language	English
Pages (from-to)	35-43
Number of pages	9
Journal	Virus Genes
Volume	25
Issue number	1
DOIs	https://doi.org/10.1023/A:1020118007875
Publication status	Published - 2002
Externally published	Yes

Keywords

Genetic organization
Nucleopolyhedrovirus
ORF analysis
TnSNPV
lef-6
p74

ASJC Scopus subject areas

Molecular Biology
Genetics
Virology

Access to Document

10.1023/A:1020118007875

Cite this

@article{892707cb87c74902a4df28a854aa311f,

title = "The genetic organization of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni",

abstract = "In order to investigate the genomic organization of the Trichoplusia ni Single Capsid Nucleopolyhedrovirus (TnSNPV), a 2,966 basepairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading frames (ORFs) homologous to baculovirus genes, including p26, fibrillin (p10), AcMNPV ORF-29, late expression factor 6 (lef-6) and the C-terminal portion of p74, on either strand of DNA. Predicted amino acid sequences for the ORFs were compared and identity values of between 12% and 54% were observed. TnSNPV has previously been tentatively identified as a member of the Group II NPVs. Clustering and arrangement of the TnSNPV genes were similar to the clustering reported for SeMNPV, confirming TnSNPV as a Group II NPV.",

keywords = "Genetic organization, Nucleopolyhedrovirus, ORF analysis, TnSNPV, lef-6, p74",

author = "Fielding, {Burtram Clinton} and Sehaam Khan and Weizhou Wang and Courtney Kruger and Rayaana Abrahams and Sean Davison",

year = "2002",

doi = "10.1023/A:1020118007875",

language = "English",

volume = "25",

pages = "35--43",

journal = "Virus Genes",

issn = "0920-8569",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - The genetic organization of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni

AU - Fielding, Burtram Clinton

AU - Khan, Sehaam

AU - Wang, Weizhou

AU - Kruger, Courtney

AU - Abrahams, Rayaana

AU - Davison, Sean

PY - 2002

Y1 - 2002

N2 - In order to investigate the genomic organization of the Trichoplusia ni Single Capsid Nucleopolyhedrovirus (TnSNPV), a 2,966 basepairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading frames (ORFs) homologous to baculovirus genes, including p26, fibrillin (p10), AcMNPV ORF-29, late expression factor 6 (lef-6) and the C-terminal portion of p74, on either strand of DNA. Predicted amino acid sequences for the ORFs were compared and identity values of between 12% and 54% were observed. TnSNPV has previously been tentatively identified as a member of the Group II NPVs. Clustering and arrangement of the TnSNPV genes were similar to the clustering reported for SeMNPV, confirming TnSNPV as a Group II NPV.

AB - In order to investigate the genomic organization of the Trichoplusia ni Single Capsid Nucleopolyhedrovirus (TnSNPV), a 2,966 basepairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading frames (ORFs) homologous to baculovirus genes, including p26, fibrillin (p10), AcMNPV ORF-29, late expression factor 6 (lef-6) and the C-terminal portion of p74, on either strand of DNA. Predicted amino acid sequences for the ORFs were compared and identity values of between 12% and 54% were observed. TnSNPV has previously been tentatively identified as a member of the Group II NPVs. Clustering and arrangement of the TnSNPV genes were similar to the clustering reported for SeMNPV, confirming TnSNPV as a Group II NPV.

KW - Genetic organization

KW - Nucleopolyhedrovirus

KW - ORF analysis

KW - TnSNPV

KW - lef-6

KW - p74

UR - http://www.scopus.com/inward/record.url?scp=0036348716&partnerID=8YFLogxK

U2 - 10.1023/A:1020118007875

DO - 10.1023/A:1020118007875

M3 - Article

C2 - 12206306

AN - SCOPUS:0036348716

SN - 0920-8569

VL - 25

SP - 35

EP - 43

JO - Virus Genes

JF - Virus Genes

IS - 1

ER -

The genetic organization of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this