The effect of low level laser irradiation on adult human adipose derived stem cells

B. Mvula, T. Mathope, T. Moore, H. Abrahamse

Research output: Contribution to journalArticlepeer-review

127 Citations (Scopus)

Abstract

This study investigated the effect of low level laser irradiation on primary cultures of adult human adipose derived stem cells (ADSC) using a 635-nm diode laser, at 5 J/cm2 with a power output of 50.2 mW and a power density of 5.5 mW/cm2. Cellular morphology did not appear to change after irradiation. Using the trypan blue exclusion test, the cellular viability of irradiated cells increased by 1% at 24 h and 1.6% at 48 h but was not statistically significant. However, the increase of cellular viability as measured by ATP luminescence was statistically significant at 48 h (p<0.05). Proliferation of irradiated cells, measured by optical density, resulted in statistically significant increases in values compared to nonirradiated cells (p<0.05) at both time points. Western blot analysis and immunocytochemical labeling indicated an increase in the expression of stem cell marker β1-integrin after irradiation. These results indicate that 5 J/cm 2 of laser irradiation can positively affect human adipose stem cells by increasing cellular viability, proliferation, and expression of β1-integrin.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalLasers in Medical Science
Volume23
Issue number3
DOIs
Publication statusPublished - Jul 2008

Keywords

  • 635 nm diode laser
  • ATP luminescence
  • Primary human adipocyte stem cells
  • Trypan blue test viability
  • β1-integrin differentiation expression

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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