The effect of laser irradiation on adipose derived stem cell proliferation and differentiation

H. Abrahamse, J. De Villiers, B. Mvula

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

There are two fundamental types of stem cells: Embryonic Stem cells and Adult Stem cells. Adult Stem cells have a more restricted potential and can usually differentiate into a few different cell types. In the body these cells facilitate the replacement or repair of damaged or diseased cells in organs. Low intensity laser irradiation was shown to increase stem cell migration and stimulate proliferation and it is thought that treatment of these cells with laser irradiation may increase the stem cell harvest and have a positive effect on the viability and proliferation. Our research is aimed at determining the effect of laser irradiation on differentiation of Adipose Derived Stem Cells (ADSCs) into different cell types using a diode laser with a wavelength of 636 nm and at 5 J/cm2. Confirmation of stem cell characteristics and well as subsequent differentiation were assessed using Western blot analysis and cellular morphology supported by fluorescent live cell imaging. Functionality of subsequent differentiated cells was confirmed by measuring adenosine triphosphate (ATP) production and cell viability.

Original languageEnglish
Title of host publicationLaser Florence 2008 - A Bridge to the Laser Medicine World
Subtitle of host publicationSelected Presentations at the International Laser Medicine Congress
Pages53-63
Number of pages11
DOIs
Publication statusPublished - 2009
EventInternational Laser Medicine Congress, Laser Florence 2008: A Bridge To The Laser Medicine World - Firenze, Italy
Duration: 31 Oct 20081 Nov 2008

Publication series

NameAIP Conference Proceedings
Volume1142
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

ConferenceInternational Laser Medicine Congress, Laser Florence 2008: A Bridge To The Laser Medicine World
Country/TerritoryItaly
CityFirenze
Period31/10/081/11/08

Keywords

  • Human adult adipose derived stem cells
  • Laser
  • Proliferation

ASJC Scopus subject areas

  • General Physics and Astronomy

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