The effect of femtosecond laser irradiation on the growth kinetics of Staphylococcus aureus: An in vitro study

Esraa Ahmed, Ahmed O. El-Gendy, Michael R. Hamblin, Tarek Mohamed

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We investigated the effect of femtosecond laser irradiation on the growth kinetics of Staphylococcus aureus. In order to improve laser-based antimicrobial therapy and develop a clinically viable modality, various laser parameters such as laser light wavelength, laser power, exposure time, and energy density were studied. The INSPIRE HF100 laser system (Spectra Physics) provided the femtosecond laser light, which was pumped by a mode-locked femtosecond Ti: sapphire laser MAI TAI HP (Spectra Physics). The survival of the bacterial cells was monitored after irradiation by determination of growth rate using optical density, which is a rapid, simple, and reliable method. The growth rate of laser-exposed cultures was compared to control cultures. Fifteen minutes of exposure to femtosecond laser radiation with a wavelength of 390 nm and 400 nm at an average power of 50 mW was enough to significantly reduce bacterial viability, with a lag in the growth phase of 5 h longer than the control culture (P < 0.0001 by ANOVA and Tukey test).

Original languageEnglish
Article number112240
JournalJournal of Photochemistry and Photobiology B: Biology
Volume221
DOIs
Publication statusPublished - Aug 2021

Keywords

  • Antibacterial activity
  • Bacterial growth kinetics
  • Femtosecond laser
  • Laser parameters
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology, Nuclear Medicine and Imaging

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