The antimicrobial effect of 400 nm femtosecond laser and silver nanoparticles on gram-positive and gram-negative bacteria

Ahmed O. El-Gendy, Ahmed Samir, Esraa Ahmed, Chukuka S. Enwemeka, Tarek Mohamed

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Silver nanoparticles are well-known for their antimicrobial effect. However, they are potentially toxic in high doses. We explored the possibility of enhancing the bactericidal effect of low concentrations of silver nanoparticles with blue light femtosecond laser irradiation, since such concentrations are less toxic. The growth dynamics of Pseudomonas aeruginosa, Listeria monocytogenes and methicillin-resistant Staphylococcus aureus grown in pre-synthesized silver nanoparticles were measured with or without pre-irradiation with 50 mW and 400 nm femtosecond laser irradiation. With each bacterium, combined treatment with laser and silver nanoparticles significantly reduced bacterial growth, indicating that this form of treatment could be beneficial in the ongoing efforts to reduce the deleterious effects of antibiotic resistant Gram-positive and Gram-negative bacteria. The combined treatment was more antimicrobial than treatment with silver nanoparticles alone or photo-irradiation alone. P. aeruginosa and L. monocytogenes were more susceptible to the bactericidal effects of silver nanoparticles, and the combination of laser treatment and silver nanoparticles than MRSA.

Original languageEnglish
Article number112300
JournalJournal of Photochemistry and Photobiology B: Biology
Volume223
DOIs
Publication statusPublished - Oct 2021
Externally publishedYes

Keywords

  • Antimicrobial blue light
  • Femtosecond laser ablation
  • Listeria monocytogenes
  • Pseudomonas aeruginosa
  • Silver nanoparticles
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology, Nuclear Medicine and Imaging

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