Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe3O4 Nanoparticles

Shobana Nagaraj; Carlo Berrino; Aletta Prinsloo; Pankaj Mohanty; Ramesh Pandian; Clement Penny; Charles Sheppard

doi:10.1007/978-981-96-3624-2_6

Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe₃O₄ Nanoparticles

Shobana Nagaraj
, Carlo Berrino
, Aletta Prinsloo
, Pankaj Mohanty
, Ramesh Pandian
, Clement Penny
, Charles Sheppard

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

In this contribution, the characteristics and anticancer activity of chromium (Cr) doped Fe₃O₄ nanoparticles, of the form Fe²⁺(Fe³⁺_0.95Cr³⁺_0.05)₂O₄, with 0 < x < 0.05 is discussed. The Cr-doped Fe₃O₄ nanoparticles were synthesized using the green synthesis method, employing extract derived from the Krantz aloe (Aloe arborescens) plant that is indigenous to Southern Africa. For comparison, undoped Fe₃O₄ nanoparticles were also prepared in a similar fashion, as to study the impact of the Cr doping on the impact of the compounds’ anticancer activities. Characterization, done using x-ray diffraction (XRD), confirmed the nanoparticles’ purity and crystalline nature. Transmission electron microscopy (TEM) gives particle sizes of 18 ± 1 nm and 6 ± 1 nm for the Fe₃O₄ and Cr-doped Fe₃O₄, respectively. Magnetic characterization indicates the ferrimagnetic behaviour of the particles. Fourier-transform infrared spectroscopy (FTIR) is used to probe the bonding environment of the ions. The results of cell viability assays proves that the green synthesized Fe₃O₄ and Cr-doped Fe₃O₄ nanoparticles exhibited anticancer activity when tested against colorectal adenocarcinoma (DLD-1) cells; as well as the ability to destroy more cancerous cells compared to healthy human embryonic kidney (HEK-293) cells. Present results indicate that the Cr-doped Fe₃O₄ nanoparticles were smaller in size and showed enhanced anticancer activity when compared to the undoped Fe₃O₄ nanoparticles.

Original language	English
Title of host publication	Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology
Editors	Ching Yern Chee, Cong Wang
Publisher	Springer Science and Business Media Deutschland GmbH
Pages	56-75
Number of pages	20
ISBN (Print)	9789819636235
DOIs	https://doi.org/10.1007/978-981-96-3624-2_6
Publication status	Published - 2025
Event	8th International Conference on Material Engineering and Nanotechnology, ICMEN 2024 - Kuala Lumpur, Malaysia Duration: 28 Sept 2024 → 29 Sept 2024

Publication series

Name	Springer Proceedings in Physics
Volume	421 SPPHY
ISSN (Print)	0930-8989
ISSN (Electronic)	1867-4941

Conference

Conference	8th International Conference on Material Engineering and Nanotechnology, ICMEN 2024
Country/Territory	Malaysia
City	Kuala Lumpur
Period	28/09/24 → 29/09/24

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell viability
Cr-doped FeO
Green synthesis
Krantz aloe (Aloe arborescens)

ASJC Scopus subject areas

General Physics and Astronomy

Access to Document

10.1007/978-981-96-3624-2_6

Cite this

Nagaraj, S., Berrino, C., Prinsloo, A., Mohanty, P., Pandian, R., Penny, C., & Sheppard, C. (2025). Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe₃O₄ Nanoparticles. In C. Y. Chee, & C. Wang (Eds.), Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology (pp. 56-75). (Springer Proceedings in Physics; Vol. 421 SPPHY). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/978-981-96-3624-2_6

Nagaraj, Shobana ; Berrino, Carlo ; Prinsloo, Aletta et al. / Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe₃O₄ Nanoparticles. Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology. editor / Ching Yern Chee ; Cong Wang. Springer Science and Business Media Deutschland GmbH, 2025. pp. 56-75 (Springer Proceedings in Physics).

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title = "Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe3O4 Nanoparticles",

abstract = "In this contribution, the characteristics and anticancer activity of chromium (Cr) doped Fe3O4 nanoparticles, of the form Fe2+(Fe3+0.95Cr3+0.05)2O4, with 0 < x < 0.05 is discussed. The Cr-doped Fe3O4 nanoparticles were synthesized using the green synthesis method, employing extract derived from the Krantz aloe (Aloe arborescens) plant that is indigenous to Southern Africa. For comparison, undoped Fe3O4 nanoparticles were also prepared in a similar fashion, as to study the impact of the Cr doping on the impact of the compounds{\textquoteright} anticancer activities. Characterization, done using x-ray diffraction (XRD), confirmed the nanoparticles{\textquoteright} purity and crystalline nature. Transmission electron microscopy (TEM) gives particle sizes of 18 ± 1 nm and 6 ± 1 nm for the Fe3O4 and Cr-doped Fe3O4, respectively. Magnetic characterization indicates the ferrimagnetic behaviour of the particles. Fourier-transform infrared spectroscopy (FTIR) is used to probe the bonding environment of the ions. The results of cell viability assays proves that the green synthesized Fe3O4 and Cr-doped Fe3O4 nanoparticles exhibited anticancer activity when tested against colorectal adenocarcinoma (DLD-1) cells; as well as the ability to destroy more cancerous cells compared to healthy human embryonic kidney (HEK-293) cells. Present results indicate that the Cr-doped Fe3O4 nanoparticles were smaller in size and showed enhanced anticancer activity when compared to the undoped Fe3O4 nanoparticles.",

keywords = "Cell viability, Cr-doped FeO, Green synthesis, Krantz aloe (Aloe arborescens)",

author = "Shobana Nagaraj and Carlo Berrino and Aletta Prinsloo and Pankaj Mohanty and Ramesh Pandian and Clement Penny and Charles Sheppard",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2025.; 8th International Conference on Material Engineering and Nanotechnology, ICMEN 2024 ; Conference date: 28-09-2024 Through 29-09-2024",

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Nagaraj, S, Berrino, C, Prinsloo, A , Mohanty, P, Pandian, R, Penny, C & Sheppard, C 2025, Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe₃O₄ Nanoparticles. in CY Chee & C Wang (eds), Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology. Springer Proceedings in Physics, vol. 421 SPPHY, Springer Science and Business Media Deutschland GmbH, pp. 56-75, 8th International Conference on Material Engineering and Nanotechnology, ICMEN 2024, Kuala Lumpur, Malaysia, 28/09/24. https://doi.org/10.1007/978-981-96-3624-2_6

Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe₃O₄ Nanoparticles. / Nagaraj, Shobana; Berrino, Carlo; Prinsloo, Aletta et al.
Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology. ed. / Ching Yern Chee; Cong Wang. Springer Science and Business Media Deutschland GmbH, 2025. p. 56-75 (Springer Proceedings in Physics; Vol. 421 SPPHY).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe3O4 Nanoparticles

AU - Nagaraj, Shobana

AU - Berrino, Carlo

AU - Prinsloo, Aletta

AU - Mohanty, Pankaj

AU - Pandian, Ramesh

AU - Penny, Clement

AU - Sheppard, Charles

PY - 2025

Y1 - 2025

N2 - In this contribution, the characteristics and anticancer activity of chromium (Cr) doped Fe3O4 nanoparticles, of the form Fe2+(Fe3+0.95Cr3+0.05)2O4, with 0 < x < 0.05 is discussed. The Cr-doped Fe3O4 nanoparticles were synthesized using the green synthesis method, employing extract derived from the Krantz aloe (Aloe arborescens) plant that is indigenous to Southern Africa. For comparison, undoped Fe3O4 nanoparticles were also prepared in a similar fashion, as to study the impact of the Cr doping on the impact of the compounds’ anticancer activities. Characterization, done using x-ray diffraction (XRD), confirmed the nanoparticles’ purity and crystalline nature. Transmission electron microscopy (TEM) gives particle sizes of 18 ± 1 nm and 6 ± 1 nm for the Fe3O4 and Cr-doped Fe3O4, respectively. Magnetic characterization indicates the ferrimagnetic behaviour of the particles. Fourier-transform infrared spectroscopy (FTIR) is used to probe the bonding environment of the ions. The results of cell viability assays proves that the green synthesized Fe3O4 and Cr-doped Fe3O4 nanoparticles exhibited anticancer activity when tested against colorectal adenocarcinoma (DLD-1) cells; as well as the ability to destroy more cancerous cells compared to healthy human embryonic kidney (HEK-293) cells. Present results indicate that the Cr-doped Fe3O4 nanoparticles were smaller in size and showed enhanced anticancer activity when compared to the undoped Fe3O4 nanoparticles.

AB - In this contribution, the characteristics and anticancer activity of chromium (Cr) doped Fe3O4 nanoparticles, of the form Fe2+(Fe3+0.95Cr3+0.05)2O4, with 0 < x < 0.05 is discussed. The Cr-doped Fe3O4 nanoparticles were synthesized using the green synthesis method, employing extract derived from the Krantz aloe (Aloe arborescens) plant that is indigenous to Southern Africa. For comparison, undoped Fe3O4 nanoparticles were also prepared in a similar fashion, as to study the impact of the Cr doping on the impact of the compounds’ anticancer activities. Characterization, done using x-ray diffraction (XRD), confirmed the nanoparticles’ purity and crystalline nature. Transmission electron microscopy (TEM) gives particle sizes of 18 ± 1 nm and 6 ± 1 nm for the Fe3O4 and Cr-doped Fe3O4, respectively. Magnetic characterization indicates the ferrimagnetic behaviour of the particles. Fourier-transform infrared spectroscopy (FTIR) is used to probe the bonding environment of the ions. The results of cell viability assays proves that the green synthesized Fe3O4 and Cr-doped Fe3O4 nanoparticles exhibited anticancer activity when tested against colorectal adenocarcinoma (DLD-1) cells; as well as the ability to destroy more cancerous cells compared to healthy human embryonic kidney (HEK-293) cells. Present results indicate that the Cr-doped Fe3O4 nanoparticles were smaller in size and showed enhanced anticancer activity when compared to the undoped Fe3O4 nanoparticles.

KW - Cell viability

KW - Cr-doped FeO

KW - Green synthesis

KW - Krantz aloe (Aloe arborescens)

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U2 - 10.1007/978-981-96-3624-2_6

DO - 10.1007/978-981-96-3624-2_6

M3 - Conference contribution

AN - SCOPUS:105002401509

SN - 9789819636235

T3 - Springer Proceedings in Physics

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BT - Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology

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PB - Springer Science and Business Media Deutschland GmbH

T2 - 8th International Conference on Material Engineering and Nanotechnology, ICMEN 2024

Y2 - 28 September 2024 through 29 September 2024

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Nagaraj S, Berrino C, Prinsloo A , Mohanty P, Pandian R, Penny C et al. Targeting Colorectal Cancer Using Krantz Aloe (Aloe arborescens) Extract Green Synthesised Chromium Doped Fe₃O₄ Nanoparticles. In Chee CY, Wang C, editors, Selected Articles from the 8th International Conference on Materials Engineering and Nanotechnology, ICMEN 2024 - Exploring the Frontiers of Materials and Nanotechnology. Springer Science and Business Media Deutschland GmbH. 2025. p. 56-75. (Springer Proceedings in Physics). doi: 10.1007/978-981-96-3624-2_6