Synthesis of nir-ii absorbing gelatin stabilized gold nanorods and its photothermal therapy application against fibroblast histiocytoma cells

Adewale Oladipo, Thabang Calvin Lebepe, Sundararajan Parani, Rodney Maluleke, Vuyelwa Ncapayi, Grace It Mwad Mbaz, Sandile Phinda Songca, Tetsuya Kodama, Oluwatobi Samuel Oluwafemi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompatible gelatin stabilized Au-NRs (gelatin@Au-NRs) to address this issue. The optical and structural properties of the as-synthesized gelatin@Au-NRs were investigated by Zetasizer, Ultraviolet-Visible-Near Infrared (UV-Vis-NIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The as-synthesized gelatin@Au-NRs were highly crystalline and rod-like in shape with an average length and diameter of 66.2 ± 2.3 nm and 10 ± 1.6 nm, respectively. The as-synthesized gelatin@Au-NRs showed high stability in common biological media (phosphate buffer saline and Dulbecco’s Modified Eagle’s Medium) compared to CTAB capped Au-NRs. Similarly, the gelatin@Au-NRs showed an improved heat production and outstanding cell viability against two different cancer cell lines; KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) compared to CTAB capped Au-NRs and PEG@Au-NRs. An in vitro photothermal therapy study against KM-Luc/GFP showed that gelatin@Au-NRs effectively destroys the cancer cells.

Original languageEnglish
Article number1137
JournalPharmaceuticals
Volume14
Issue number11
DOIs
Publication statusPublished - Nov 2021

Keywords

  • Cytotoxicity
  • Gelatin
  • Gold nanorods
  • Near-infrared
  • Photothermal therapy
  • Stability

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

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