TY - JOUR
T1 - Synthesis and characterization of gold nanoparticles biosynthesised from Aspalathus linearis (Burm.f.) R.Dahlgren For progressive macular hypomelanosis
AU - Blom van Staden, Analike
AU - Kovacs, Daniela
AU - Cardinali, Giorgia
AU - Picardo, Mauro
AU - Lebeko, Maribanyana
AU - Khumalo, Nonhlanhla C.
AU - Ray, Suprakas Sinha
AU - Lall, Namrita
N1 - Publisher Copyright:
© 2021 Elsevier GmbH
PY - 2021/10
Y1 - 2021/10
N2 - Introduction: The study investigated a natural solution for the hypopigmentary disorder, progressive macular hypomelanosis (PMH). The aim was to determine whether gold nanoparticles synthesized from Aspalathus linearis (Burm.f.) R.Dahlgren could be effective for PMH. Methods Gold nanoparticles (ALAuNPs) were synthesized using the ethanolic extract of A. linearis (ALEtOH) and tested, along with the extract for its regulatory effect of melanogenesis in human melanocytes and its antibacterial activity against Cutibacterium acnes (ATCC 6919 strain). Melanosome transfer was determined in a co-culture of normal human melanocytes and keratinocytes and was quantified using flow cytometry. Results: The ALAuNPstab (AuNPs stabilized with gum arabic) and ALAuNPnon-stab (non-stabilized) exhibited significantly (P < 0.001) higher antibacterial activity against C. acnes with 50 % inhibition at a concentration of 66.05 ± 1.10 μg/mL and 68.12 ± 1.05 μg/mL, respectively, as compared to that of ALEtOH (271.20 ± 2.32 μg/mL). The ALEtOH exhibited 50 % reduction in cell proliferation (IC50 value) in human melanocytes at 345.5 ± 2.47 μg/mL, whilst ALAuNPstab and ALAuNPnon-stab exhibited an IC50 value of 67.51 ± 1.12 and 69.63 ± 1.07 μg/mL, respectively. The ALEtOH and ALAuNPstab tested at a concentration of 62.5 μg/mL, exhibited a significant increase (P < 0.001) in the total number of melanin present in human melanocytes compared to the untreated (control) cells. The cells treated with ALEtOH and ALAuNPstab led to a significant (P < 0.001) increase in the number of melanosomes transferred as compared to the untreated cells. Conclusion: This study confirmed that A. linearis and its biosynthesised gold nanoparticles stimulated melanin synthesis.
AB - Introduction: The study investigated a natural solution for the hypopigmentary disorder, progressive macular hypomelanosis (PMH). The aim was to determine whether gold nanoparticles synthesized from Aspalathus linearis (Burm.f.) R.Dahlgren could be effective for PMH. Methods Gold nanoparticles (ALAuNPs) were synthesized using the ethanolic extract of A. linearis (ALEtOH) and tested, along with the extract for its regulatory effect of melanogenesis in human melanocytes and its antibacterial activity against Cutibacterium acnes (ATCC 6919 strain). Melanosome transfer was determined in a co-culture of normal human melanocytes and keratinocytes and was quantified using flow cytometry. Results: The ALAuNPstab (AuNPs stabilized with gum arabic) and ALAuNPnon-stab (non-stabilized) exhibited significantly (P < 0.001) higher antibacterial activity against C. acnes with 50 % inhibition at a concentration of 66.05 ± 1.10 μg/mL and 68.12 ± 1.05 μg/mL, respectively, as compared to that of ALEtOH (271.20 ± 2.32 μg/mL). The ALEtOH exhibited 50 % reduction in cell proliferation (IC50 value) in human melanocytes at 345.5 ± 2.47 μg/mL, whilst ALAuNPstab and ALAuNPnon-stab exhibited an IC50 value of 67.51 ± 1.12 and 69.63 ± 1.07 μg/mL, respectively. The ALEtOH and ALAuNPstab tested at a concentration of 62.5 μg/mL, exhibited a significant increase (P < 0.001) in the total number of melanin present in human melanocytes compared to the untreated (control) cells. The cells treated with ALEtOH and ALAuNPstab led to a significant (P < 0.001) increase in the number of melanosomes transferred as compared to the untreated cells. Conclusion: This study confirmed that A. linearis and its biosynthesised gold nanoparticles stimulated melanin synthesis.
KW - Aspalathus linearis
KW - Cutibacterium acnes
KW - Gold nanoparticles
KW - Melanin transfer
KW - Progressive macular hypomelanosis
UR - http://www.scopus.com/inward/record.url?scp=85111031140&partnerID=8YFLogxK
U2 - 10.1016/j.hermed.2021.100481
DO - 10.1016/j.hermed.2021.100481
M3 - Article
AN - SCOPUS:85111031140
SN - 2210-8033
VL - 29
JO - Journal of Herbal Medicine
JF - Journal of Herbal Medicine
M1 - 100481
ER -