TY - JOUR
T1 - Synthesis and application of silver nanoparticles from lemon peel extract and other local plant extracts for detection of “blood” at crime scene
AU - Mojela, Nare Leah
AU - Pillay, Kriveshini
AU - Komane, Patrick Phogothi
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/12
Y1 - 2024/12
N2 - This study reports the enhancement of luminol's sensitivity for blood detection at a crime scene. Silver nanoparticles (AgNPs) were synthesized from the extracts derived from Citrus limon peels, Aloe vera leaves, Capsicum annuum barks from Salix alba, Crinum asiaticum Linn leaves, and Crinum macowanii bulb using green chemistry procedure. Synthesis of silver nanoparticles followed a green chemistry method utilizing water as a solvent, with the phytochemicals from the extracts acting as stabilizing and reducing agents to reduce Ag(I) to Ag(0). The synthesized AgNPs were characterized using Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Ultraviolet-visible (UV-Vis). XRD revealed that the structural composition of silver nanoparticles (AgNPs) was face-centered cubic crystalline (Fcc). TEM imaging demonstrated the spherical crystalline nature of silver nanoparticles (AgNPs) derived from the plant extracts. These silver nanoparticles (AgNPs) were used to enhance the shelf-life, chemiluminescence intensity, specificity, and sensitivity of luminol. It was observed that the silver nanoparticles synthesized from these plant extracts effectively enhanced the chemiluminescence signal of luminol. Moreover, applying plant extracts in blood detection demonstrated a higher fluorescence when exposed to ultraviolet (UV) light.
AB - This study reports the enhancement of luminol's sensitivity for blood detection at a crime scene. Silver nanoparticles (AgNPs) were synthesized from the extracts derived from Citrus limon peels, Aloe vera leaves, Capsicum annuum barks from Salix alba, Crinum asiaticum Linn leaves, and Crinum macowanii bulb using green chemistry procedure. Synthesis of silver nanoparticles followed a green chemistry method utilizing water as a solvent, with the phytochemicals from the extracts acting as stabilizing and reducing agents to reduce Ag(I) to Ag(0). The synthesized AgNPs were characterized using Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Ultraviolet-visible (UV-Vis). XRD revealed that the structural composition of silver nanoparticles (AgNPs) was face-centered cubic crystalline (Fcc). TEM imaging demonstrated the spherical crystalline nature of silver nanoparticles (AgNPs) derived from the plant extracts. These silver nanoparticles (AgNPs) were used to enhance the shelf-life, chemiluminescence intensity, specificity, and sensitivity of luminol. It was observed that the silver nanoparticles synthesized from these plant extracts effectively enhanced the chemiluminescence signal of luminol. Moreover, applying plant extracts in blood detection demonstrated a higher fluorescence when exposed to ultraviolet (UV) light.
KW - Chemiluminescence
KW - fluorescence
KW - forensic science
KW - Green chemistry
KW - Plant extract
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85207795349&partnerID=8YFLogxK
U2 - 10.1016/j.forsciint.2024.112257
DO - 10.1016/j.forsciint.2024.112257
M3 - Article
AN - SCOPUS:85207795349
SN - 0379-0738
VL - 365
JO - Forensic Science International
JF - Forensic Science International
M1 - 112257
ER -