Susceptibility of Cryptococcus neoformans to photodynamic inactivation is associated with cell wall integrity

Beth Burgwyn Fuchs, George P. Tegos, Michael R. Hamblin, Eleftherios Mylonakis

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Photodynamic therapy is a rapidly developing antimicrobial technology which combines a nontoxic photoactivatable dye or photosensitizer with harmless visible light of the correct wavelength to excite the dye to its reactive triplet state to generate reactive oxygen species toxic to cells. In this report we present evidence that the fungal pathogen Cryptococcus neoformans is susceptible to photodynamic inactivation by use of a polycationic conjugate of polyethyleneimine and the photosensitizer chlorin(e6). A C. neoformans rom2 mutant, with a mutation involving a putative Rho1 guanyl nucleotide exchange factor that is part of the protein kinase C-cell wall integrity pathway, demonstrated a compromised cell wall and less (1,3)β-D glucan than the wild-type strain and increased accumulation of PEI-ce6 as assessed by fluorescence uptake and confocal microscopy. Interestingly, C. neoformans rom2 was hypersusceptible to photodynamic inactivation and coincubation of wild-type C. neoformans strain KN99α with caspofungin-enhanced photoinactivation. These studies demonstrated that C. neoformans is sensitive to photodynamic therapy and illustrated the significance of cell wall integrity in microbial susceptibility to antimicrobial photodynamic inactivation.

Original languageEnglish
Pages (from-to)2929-2936
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume51
Issue number8
DOIs
Publication statusPublished - Aug 2007
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Susceptibility of Cryptococcus neoformans to photodynamic inactivation is associated with cell wall integrity'. Together they form a unique fingerprint.

Cite this