Studying Plant Specialized Metabolites Using Computational Metabolomics Strategies

Lana Mutabdžija, Akhona Myoli, Niek F. de Jonge, Tito Damiani, Robin Schmid, Justin J.J. van der Hooft, Fidele Tugizimana, Tomáš Pluskal

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Plant specialized metabolites have diversified vastly over the course of plant evolution, and they are considered key players in complex interactions between plants and their environment. The chemical diversity of these metabolites has been widely explored and utilized in agriculture and crop enhancement, the food industry, and drug development, among other areas. However, the immensity of the plant metabolome can make its exploration challenging. Here we describe a protocol for exploring plant specialized metabolites that combines high-resolution mass spectrometry and computational metabolomics strategies, including molecular networking, identification of structural motifs, as well as prediction of chemical structures and metabolite classes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages97-136
Number of pages40
DOIs
Publication statusPublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2788
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • GNPS
  • Molecular networking
  • MS2LDA
  • MS2Query
  • MZmine
  • Plant metabolomics
  • SIRIUS
  • Specialized metabolites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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