Structural membrane changes induced by pulsed blue light on methicillin-resistant Staphylococcus aureus (MRSA)

Chynna Bowman; Violet V. Bumah; Ingrid R. Niesman; Paulina Cortez; Chukuka S. Enwemeka

doi:10.1016/j.jphotobiol.2021.112150

Structural membrane changes induced by pulsed blue light on methicillin-resistant Staphylococcus aureus (MRSA)

Chynna Bowman, Violet V. Bumah, Ingrid R. Niesman, Paulina Cortez, Chukuka S. Enwemeka

San Diego State University

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Background: In a recent study we showed that blue light inactivates methicillin-resistant Staphylococcus aureus (MRSA) by perturbing, depolarizing, and disrupting its cell membrane. Purpose: The current study presents visual evidence that the observed biochemical changes also result in cell metabolic changes and structural alteration of the cell membrane. Methods: Cultures of MRSA were treated with 450 nm pulsed blue light (PBL) at 3 mW/cm² irradiance, using a sub lethal dose of 2.7 J/cm² radiant exposure three times at 30-min intervals. Following 24 h incubation at 37 °C, irradiated colonies and control non-irradiated colonies were processed for light and transmission electron microscopy. Results: The images obtained revealed three major effects of PBL; (1) disruption of MRSA cell membrane, (2) alteration of membrane structure, and (3) disruption of cell replication. Conclusion: These signs of bacterial inactivation at a dose deliberately selected to be sub-lethal supports our previous finding that rapid depolarization of bacterial cell membrane and disruption of cellular function comprise another mechanism underlying photo-inactivation of bacteria. Further, it affirms the potency of PBL.

Original language	English
Article number	112150
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	216
DOIs	https://doi.org/10.1016/j.jphotobiol.2021.112150
Publication status	Published - Mar 2021
Externally published	Yes

Keywords

Electron microscopy
MRSA
Photobiomodulation
Pulsed blue light

ASJC Scopus subject areas

Radiation
Radiological and Ultrasound Technology
Biophysics
Radiology, Nuclear Medicine and Imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jphotobiol.2021.112150

Cite this

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title = "Structural membrane changes induced by pulsed blue light on methicillin-resistant Staphylococcus aureus (MRSA)",

abstract = "Background: In a recent study we showed that blue light inactivates methicillin-resistant Staphylococcus aureus (MRSA) by perturbing, depolarizing, and disrupting its cell membrane. Purpose: The current study presents visual evidence that the observed biochemical changes also result in cell metabolic changes and structural alteration of the cell membrane. Methods: Cultures of MRSA were treated with 450 nm pulsed blue light (PBL) at 3 mW/cm2 irradiance, using a sub lethal dose of 2.7 J/cm2 radiant exposure three times at 30-min intervals. Following 24 h incubation at 37 °C, irradiated colonies and control non-irradiated colonies were processed for light and transmission electron microscopy. Results: The images obtained revealed three major effects of PBL; (1) disruption of MRSA cell membrane, (2) alteration of membrane structure, and (3) disruption of cell replication. Conclusion: These signs of bacterial inactivation at a dose deliberately selected to be sub-lethal supports our previous finding that rapid depolarization of bacterial cell membrane and disruption of cellular function comprise another mechanism underlying photo-inactivation of bacteria. Further, it affirms the potency of PBL.",

keywords = "Electron microscopy, MRSA, Photobiomodulation, Pulsed blue light",

author = "Chynna Bowman and Bumah, {Violet V.} and Niesman, {Ingrid R.} and Paulina Cortez and Enwemeka, {Chukuka S.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = mar,

doi = "10.1016/j.jphotobiol.2021.112150",

language = "English",

volume = "216",

journal = "Journal of Photochemistry and Photobiology B: Biology",

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T1 - Structural membrane changes induced by pulsed blue light on methicillin-resistant Staphylococcus aureus (MRSA)

AU - Bowman, Chynna

AU - Bumah, Violet V.

AU - Niesman, Ingrid R.

AU - Cortez, Paulina

AU - Enwemeka, Chukuka S.

PY - 2021/3

Y1 - 2021/3

N2 - Background: In a recent study we showed that blue light inactivates methicillin-resistant Staphylococcus aureus (MRSA) by perturbing, depolarizing, and disrupting its cell membrane. Purpose: The current study presents visual evidence that the observed biochemical changes also result in cell metabolic changes and structural alteration of the cell membrane. Methods: Cultures of MRSA were treated with 450 nm pulsed blue light (PBL) at 3 mW/cm2 irradiance, using a sub lethal dose of 2.7 J/cm2 radiant exposure three times at 30-min intervals. Following 24 h incubation at 37 °C, irradiated colonies and control non-irradiated colonies were processed for light and transmission electron microscopy. Results: The images obtained revealed three major effects of PBL; (1) disruption of MRSA cell membrane, (2) alteration of membrane structure, and (3) disruption of cell replication. Conclusion: These signs of bacterial inactivation at a dose deliberately selected to be sub-lethal supports our previous finding that rapid depolarization of bacterial cell membrane and disruption of cellular function comprise another mechanism underlying photo-inactivation of bacteria. Further, it affirms the potency of PBL.

AB - Background: In a recent study we showed that blue light inactivates methicillin-resistant Staphylococcus aureus (MRSA) by perturbing, depolarizing, and disrupting its cell membrane. Purpose: The current study presents visual evidence that the observed biochemical changes also result in cell metabolic changes and structural alteration of the cell membrane. Methods: Cultures of MRSA were treated with 450 nm pulsed blue light (PBL) at 3 mW/cm2 irradiance, using a sub lethal dose of 2.7 J/cm2 radiant exposure three times at 30-min intervals. Following 24 h incubation at 37 °C, irradiated colonies and control non-irradiated colonies were processed for light and transmission electron microscopy. Results: The images obtained revealed three major effects of PBL; (1) disruption of MRSA cell membrane, (2) alteration of membrane structure, and (3) disruption of cell replication. Conclusion: These signs of bacterial inactivation at a dose deliberately selected to be sub-lethal supports our previous finding that rapid depolarization of bacterial cell membrane and disruption of cellular function comprise another mechanism underlying photo-inactivation of bacteria. Further, it affirms the potency of PBL.

KW - Electron microscopy

KW - MRSA

KW - Photobiomodulation

KW - Pulsed blue light

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ER -

Structural membrane changes induced by pulsed blue light on methicillin-resistant Staphylococcus aureus (MRSA)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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