Single and consecutive application of near-infrared and green irradiation modulates adipose derived stem cell proliferation and affect differentiation factors

Regenerative medicine uses undifferentiated adipose-derived mesenchymal stem cells (ADMSCs) to differentiate into multiple cell types. Photobiomodulation (PBM) is a rapidly growing treatment for pain and inflammation reduction, as well as tissue healing. PBM's efficacy is dependent on wavelength and energy dosage. Red (600–700 nm) and near-infrared (780–1100 nm) wavelengths have been shown to promote cell proliferation. Light wavelengths such as green (495 nm–570 nm) have been found to influence ADMSC differentiation. The initiation of ADMSC proliferation and differentiation requires physiologically relevant levels of reactive oxygen species (ROS), while increased levels inhibit self-renewal. Stem cell differentiation is guided by mitochondrial metabolism, where an increased mitochondrial membrane potential (MMP) is associated with higher in vitro differentiation capacity. ADMSCs must home to and accumulate at the sites of injury in regenerative medicine, so cell homing is critical. The aim of this in vitro study was to compare consecutive NIR (825 nm) and green (525 nm) applications on ADMSC morphology and physiology with the possibility that multiple wavelengths could lead to a combination of the two effects. The results showed that concurrent use of NIR-green irradiation significantly stimulated ADMSC proliferation, increasing population density and cellular ATP. Furthermore, NIR-green showed a time dependent increase in ROS production and was significantly higher at 7 days. Consecutive NIR-green irradiation significantly increased MMP and was most effective at facilitating ADMSC migration over time. Findings suggest that with consecutive NIR and green irradiation, the ADMSCs can rapidly proliferate, but can also be modulated for regenerative purposes.