Shock wave-mediated molecular delivery into cells

Tetsuya Kodama; Apostolos G. Doukas; Michael R. Hamblin

doi:10.1016/S0167-4889(01)00177-X

Shock wave-mediated molecular delivery into cells

Tetsuya Kodama, Apostolos G. Doukas, Michael R. Hamblin

Massachusetts General Hospital

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

A single shock wave generated by a shock tube is able to effectively deliver macromolecules such as fluorescein isothiocyanate-dextran into the cytoplasm of living cells without causing cytotoxicity. We report on the effect of varying the molecular weight of the dextran and the number of shock waves on the efficiency of delivery into a cancer cell line. The fraction of cells permeabilized and the total fluorescence delivered were measured by flow cytometry, and the cellular viability by a tetrazolium assay on adherent cells and these values were compared to cell permeabilization using digitonin. Shock waves can deliver molecules of up to 2 000 000 molecular weight into the cytoplasm of cells without toxicity and may have applications in gene therapy.

Original language	English
Pages (from-to)	186-194
Number of pages	9
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1542
Issue number	1-3
DOIs	https://doi.org/10.1016/S0167-4889(01)00177-X
Publication status	Published - 30 Jan 2002
Externally published	Yes

Keywords

Digitonin
FITC-dextran
Flow cytometry
Fluorescence
Membrane permeabilization
Shock tube

ASJC Scopus subject areas

Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0167-4889(01)00177-X

Cite this

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AU - Doukas, Apostolos G.

AU - Hamblin, Michael R.

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Shock wave-mediated molecular delivery into cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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