Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT

Burtram C. Fielding, Vithiagaran Gunalan, Timothy H.P. Tan, Chih Fong Chou, Shuo Shen, Sehaam Khan, Seng Gee Lim, Wanjin Hong, Yee Joo Tan

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.

Original languageEnglish
Pages (from-to)1201-1208
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number4
Publication statusPublished - 19 May 2006
Externally publishedYes


  • Accessory protein
  • ORF 7a
  • Protein-protein interaction
  • SARS-CoV
  • SGT
  • TPR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT'. Together they form a unique fingerprint.

Cite this