Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT

Burtram C. Fielding; Vithiagaran Gunalan; Timothy H.P. Tan; Chih Fong Chou; Shuo Shen; Sehaam Khan; Seng Gee Lim; Wanjin Hong; Yee Joo Tan

doi:10.1016/j.bbrc.2006.03.091

Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT

Burtram C. Fielding, Vithiagaran Gunalan, Timothy H.P. Tan, Chih Fong Chou, Shuo Shen, Sehaam Khan, Seng Gee Lim, Wanjin Hong, Yee Joo Tan

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.

Original language	English
Pages (from-to)	1201-1208
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	343
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2006.03.091
Publication status	Published - 19 May 2006
Externally published	Yes

Keywords

Accessory protein
ORF 7a
Protein-protein interaction
SARS-CoV
SGT
TPR

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2006.03.091

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title = "Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT",

abstract = "Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.",

keywords = "Accessory protein, ORF 7a, Protein-protein interaction, SARS-CoV, SGT, TPR",

author = "Fielding, {Burtram C.} and Vithiagaran Gunalan and Tan, {Timothy H.P.} and Chou, {Chih Fong} and Shuo Shen and Sehaam Khan and Lim, {Seng Gee} and Wanjin Hong and Tan, {Yee Joo}",

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doi = "10.1016/j.bbrc.2006.03.091",

language = "English",

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AU - Fielding, Burtram C.

AU - Gunalan, Vithiagaran

AU - Tan, Timothy H.P.

AU - Chou, Chih Fong

AU - Shen, Shuo

AU - Khan, Sehaam

AU - Lim, Seng Gee

AU - Hong, Wanjin

AU - Tan, Yee Joo

PY - 2006/5/19

Y1 - 2006/5/19

N2 - Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.

AB - Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.

KW - Accessory protein

KW - ORF 7a

KW - Protein-protein interaction

KW - SARS-CoV

KW - SGT

KW - TPR

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IS - 4

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Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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