Selected herbal feed additives showing protective effects against ochratoxin A toxicosis in broiler chicks

S. D. Stoev; P. Njobeh; I. Zarkov; T. Mircheva; D. Zapryanova; S. Denev; B. Dimitrova

doi:10.3920/WMJ2019.2432

Selected herbal feed additives showing protective effects against ochratoxin A toxicosis in broiler chicks

S. D. Stoev, P. Njobeh, I. Zarkov, T. Mircheva, D. Zapryanova, S. Denev, B. Dimitrova

Biotechnology and Food Technology

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The protective effects of herbal feed additives Silybum marianum, Withania somnifera and Centella asiatica against the toxic effects of ochratoxin A (OTA) were studied in 70 broiler chicks aged from 11 to 42 days. OTA was given with the feed at levels 5 mg/kg, whereas S. marianum, W. somnifera and C. asiatica were given at levels of 1,100, 4,000 and 4,600 mg/kg, respectively. All chicks were immunised at the age of 14 days against Newcastle disease. A protective effect of all studied herbal additives against the immunosuppressive effect of OTA and associated biochemical or pathomorphological changes was seen. The intensity of macroscopical and histopathological changes, the deviations in relative organs’ weight or body weight, the biochemical changes and the decrease of antibody titer were strongest in the OTA-exposed chicks without herbal supplementation; followed by chicks treated additionally with C. asiatica, whereas the same changes were significantly slighter or not seen in chicks additionally treated with the herbal additives W. somnifera or S. marianum. The slight increase in the serum levels of uric acid and the enzyme activity of aspartate transaminase and alanine transaminase also supported the protective effects of both herbs on the kidneys and/or liver. The strong immunosuppressive effect of OTA on humoral immune response against Newcastle disease was completely prevented in the chicks taking the herbal additives W. somnifera or S. marianum, which was supported by the higher relative weight of immunocompetent (lymphoid) organs in the same chicks. A hepatoprotective effect was found in OTA-exposed chicks treated additionally with W. somnifera and S. marianum, whereas a nephroprotective effect was only found in the chicks additionally treated with S. marianum as observed from the biochemical and pathomorphological findings. The same herbs could be used as a practical approach for safely utilising of OTA-contaminated feed.

Original language	English
Pages (from-to)	257-268
Number of pages	12
Journal	World Mycotoxin Journal
Volume	12
Issue number	3
DOIs	https://doi.org/10.3920/WMJ2019.2432
Publication status	Published - 2019

Keywords

Centella asiatica
Herbal additives
Ochratoxin A
Protection
Silybum marianum
Withania somnifera

ASJC Scopus subject areas

Food Science
Toxicology
Public Health, Environmental and Occupational Health

Access to Document

10.3920/WMJ2019.2432

Cite this

@article{e44535d7ec2e4b0a8b4603293049b68b,

title = "Selected herbal feed additives showing protective effects against ochratoxin A toxicosis in broiler chicks",

abstract = "The protective effects of herbal feed additives Silybum marianum, Withania somnifera and Centella asiatica against the toxic effects of ochratoxin A (OTA) were studied in 70 broiler chicks aged from 11 to 42 days. OTA was given with the feed at levels 5 mg/kg, whereas S. marianum, W. somnifera and C. asiatica were given at levels of 1,100, 4,000 and 4,600 mg/kg, respectively. All chicks were immunised at the age of 14 days against Newcastle disease. A protective effect of all studied herbal additives against the immunosuppressive effect of OTA and associated biochemical or pathomorphological changes was seen. The intensity of macroscopical and histopathological changes, the deviations in relative organs{\textquoteright} weight or body weight, the biochemical changes and the decrease of antibody titer were strongest in the OTA-exposed chicks without herbal supplementation; followed by chicks treated additionally with C. asiatica, whereas the same changes were significantly slighter or not seen in chicks additionally treated with the herbal additives W. somnifera or S. marianum. The slight increase in the serum levels of uric acid and the enzyme activity of aspartate transaminase and alanine transaminase also supported the protective effects of both herbs on the kidneys and/or liver. The strong immunosuppressive effect of OTA on humoral immune response against Newcastle disease was completely prevented in the chicks taking the herbal additives W. somnifera or S. marianum, which was supported by the higher relative weight of immunocompetent (lymphoid) organs in the same chicks. A hepatoprotective effect was found in OTA-exposed chicks treated additionally with W. somnifera and S. marianum, whereas a nephroprotective effect was only found in the chicks additionally treated with S. marianum as observed from the biochemical and pathomorphological findings. The same herbs could be used as a practical approach for safely utilising of OTA-contaminated feed.",

keywords = "Centella asiatica, Herbal additives, Ochratoxin A, Protection, Silybum marianum, Withania somnifera",

author = "Stoev, {S. D.} and P. Njobeh and I. Zarkov and T. Mircheva and D. Zapryanova and S. Denev and B. Dimitrova",

note = "Publisher Copyright: {\textcopyright} 2019 Wageningen Academic Publishers",

year = "2019",

doi = "10.3920/WMJ2019.2432",

language = "English",

volume = "12",

pages = "257--268",

journal = "World Mycotoxin Journal",

issn = "1875-0710",

publisher = "Wageningen Academic Publishers",

number = "3",

}

TY - JOUR

T1 - Selected herbal feed additives showing protective effects against ochratoxin A toxicosis in broiler chicks

AU - Stoev, S. D.

AU - Njobeh, P.

AU - Zarkov, I.

AU - Mircheva, T.

AU - Zapryanova, D.

AU - Denev, S.

AU - Dimitrova, B.

PY - 2019

Y1 - 2019

N2 - The protective effects of herbal feed additives Silybum marianum, Withania somnifera and Centella asiatica against the toxic effects of ochratoxin A (OTA) were studied in 70 broiler chicks aged from 11 to 42 days. OTA was given with the feed at levels 5 mg/kg, whereas S. marianum, W. somnifera and C. asiatica were given at levels of 1,100, 4,000 and 4,600 mg/kg, respectively. All chicks were immunised at the age of 14 days against Newcastle disease. A protective effect of all studied herbal additives against the immunosuppressive effect of OTA and associated biochemical or pathomorphological changes was seen. The intensity of macroscopical and histopathological changes, the deviations in relative organs’ weight or body weight, the biochemical changes and the decrease of antibody titer were strongest in the OTA-exposed chicks without herbal supplementation; followed by chicks treated additionally with C. asiatica, whereas the same changes were significantly slighter or not seen in chicks additionally treated with the herbal additives W. somnifera or S. marianum. The slight increase in the serum levels of uric acid and the enzyme activity of aspartate transaminase and alanine transaminase also supported the protective effects of both herbs on the kidneys and/or liver. The strong immunosuppressive effect of OTA on humoral immune response against Newcastle disease was completely prevented in the chicks taking the herbal additives W. somnifera or S. marianum, which was supported by the higher relative weight of immunocompetent (lymphoid) organs in the same chicks. A hepatoprotective effect was found in OTA-exposed chicks treated additionally with W. somnifera and S. marianum, whereas a nephroprotective effect was only found in the chicks additionally treated with S. marianum as observed from the biochemical and pathomorphological findings. The same herbs could be used as a practical approach for safely utilising of OTA-contaminated feed.

AB - The protective effects of herbal feed additives Silybum marianum, Withania somnifera and Centella asiatica against the toxic effects of ochratoxin A (OTA) were studied in 70 broiler chicks aged from 11 to 42 days. OTA was given with the feed at levels 5 mg/kg, whereas S. marianum, W. somnifera and C. asiatica were given at levels of 1,100, 4,000 and 4,600 mg/kg, respectively. All chicks were immunised at the age of 14 days against Newcastle disease. A protective effect of all studied herbal additives against the immunosuppressive effect of OTA and associated biochemical or pathomorphological changes was seen. The intensity of macroscopical and histopathological changes, the deviations in relative organs’ weight or body weight, the biochemical changes and the decrease of antibody titer were strongest in the OTA-exposed chicks without herbal supplementation; followed by chicks treated additionally with C. asiatica, whereas the same changes were significantly slighter or not seen in chicks additionally treated with the herbal additives W. somnifera or S. marianum. The slight increase in the serum levels of uric acid and the enzyme activity of aspartate transaminase and alanine transaminase also supported the protective effects of both herbs on the kidneys and/or liver. The strong immunosuppressive effect of OTA on humoral immune response against Newcastle disease was completely prevented in the chicks taking the herbal additives W. somnifera or S. marianum, which was supported by the higher relative weight of immunocompetent (lymphoid) organs in the same chicks. A hepatoprotective effect was found in OTA-exposed chicks treated additionally with W. somnifera and S. marianum, whereas a nephroprotective effect was only found in the chicks additionally treated with S. marianum as observed from the biochemical and pathomorphological findings. The same herbs could be used as a practical approach for safely utilising of OTA-contaminated feed.

KW - Centella asiatica

KW - Herbal additives

KW - Ochratoxin A

KW - Protection

KW - Silybum marianum

KW - Withania somnifera

UR - http://www.scopus.com/inward/record.url?scp=85069749602&partnerID=8YFLogxK

U2 - 10.3920/WMJ2019.2432

DO - 10.3920/WMJ2019.2432

M3 - Article

AN - SCOPUS:85069749602

SN - 1875-0710

VL - 12

SP - 257

EP - 268

JO - World Mycotoxin Journal

JF - World Mycotoxin Journal

IS - 3

ER -

Selected herbal feed additives showing protective effects against ochratoxin A toxicosis in broiler chicks

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this