Safety of 222 nm UVC Irradiation to the Surgical Site in a Rabbit Model

Tomoaki Fukui, Takahiro Niikura, Takahiro Oda, Yohei Kumabe, Aiko Nishiaki, Rena Kaigome, Hiroyuki Ohashi, Masahiro Sasaki, Tatsushi Igarashi, Keisuke Oe, Michael R. Hamblin, Ryosuke Kuroda

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


For the prevention of surgical site infection (SSI), continuous disinfection could be helpful. Short wavelength ultraviolet radiation C (UVC) is highly bactericidal but shows cytotoxicity. Radiation of UVC with a wavelength of 222 nm to the skin is considered to be safe because it only reaches the stratum corneum. However, the safety of 222 nm irradiation to the surgical field not covered with skin is unknown. The purpose of this study was to examine the safety of 222 nm UVC irradiation on a surgical field in a rabbit model. Five types of tissue were surgically exposed and irradiated with 222 or 254 nm UVC. Immunohistological assessment against cyclobutane pyrimidine dimer (CPD), an index of DNA damage by UVC, was performed. The CPD-positive cell rate was significantly higher in the 254 nm group than in the other groups in all tissues. A 222 nm group showed significantly more CPD than control in fat tissue, but no significant difference in all other tissues. In fat tissue collected 24 h after irradiation, the 254 nm group showed higher CPD than the other groups, while the 222 nm group had reduced to the control level. These data suggest that 222 nm UVC irradiation could be a new method to safely prevent SSI.

Original languageEnglish
Pages (from-to)1365-1371
Number of pages7
JournalPhotochemistry and Photobiology
Issue number6
Publication statusPublished - 1 Nov 2022

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry


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