Role of ros-mediated tgf beta activation in laser photobiomodulation

Praveen R. Arany, Aaron Chih Hao Chen, Tristan Hunt, David J. Mooney, Michael Hamblin

Research output: Contribution to journalConference articlepeer-review

6 Citations (Scopus)


The ability of laser light to modulate specific biological processes has been well documented but the precise mechanism mediating these photobiological interactions remains an area of intense investigation. We recently published the results of our clinical trial with 30 patients in an oral tooth-extraction wound healing model using a 904nm GaAs laser (Oralaser 1010, Oralia, Konstnaz, Germany), assessing healing parameters using routine histopathology and immunostaining (Arany et al Wound Rep Regen 2007, 15, 866). We observed a better organized healing response in laser irradiated oral tissues that correlated with an increased expression of TGF-betal immediately post laser irradiation. Our data suggested the source of latent TGF-betal might be from the degranulating platelets in the serum, an abundant source of in vivo latent TGF-beta, in the freshly wounded tissues. Further, we also demonstrated the ability of the low power near-infrared laser irradiation to activate the latent TGF-beta complexes in vitro at varying fluences from 10sec (0.1 J/cm 2) to 600secs (6 J/cm 2). Using serum we observed two isoforms, namely TGF-betal and TGF-beta3, were capable of being activated by laser irradiation using an isoform-specific ELISA and a reporter based (p3TP) assay system. We are presently pursuing the precise photomolecular mechanisms focusing on potential chromophores, wavelength and fluence parameters affecting the Latent TGF-beta activation process in serum. As ROS mediated TGF-beta activation has been previously demonstrated and we are also exploring the role of Laser generated-ROS in this activation process. In summary, we present evidence of a potential molecular mechanism for laser photobiomodulation in its ability to activate latent TGF-beta complexes.

Original languageEnglish
Article number71650C
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 2009
Externally publishedYes
EventMechanisms for Low-Light Therapy IV - San Jose, CA, United States
Duration: 24 Jan 200924 Jan 2009


  • Low power lasers
  • Photobiomodulation
  • Reactive Oxygen Species
  • Transforming Growth Factor-beta

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology, Nuclear Medicine and Imaging


Dive into the research topics of 'Role of ros-mediated tgf beta activation in laser photobiomodulation'. Together they form a unique fingerprint.

Cite this