Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the involvement of the ABC transporter protein

Errol Duncan Cason, Lizelle Ann Piater, Esta van Heerden

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

In this study we investigated the effect of uranium on the growth of the bacterium Thermus scotoductus strain SA-01 as well as the whole cell U(VI) reduction capabilities of the organism. Also, site-directed mutagenesis confirmed the identity of a protein capable of a possible alternative mechanism of U(VI) reduction. SA-01 can grow aerobically in up to 1.25. mM uranium and has the capability to reduce low levels of U(VI) in under 20. h. TEM analysis performed on cells exposed to uranium showed extracellular and membrane-bound accumulation of uranium. The reductase-like protein was surprisingly identified as a peptide ABC transporter, peptide-binding protein. This study showcases the concept of protein promiscuity, where this protein with a distinct function in situ can also have the unintended function of a reactant for the reduction of U(VI).

Original languageEnglish
Pages (from-to)572-577
Number of pages6
JournalChemosphere
Volume86
Issue number6
DOIs
Publication statusPublished - Feb 2012

Keywords

  • ABC transporter protein
  • Metal resistance
  • Thermophile
  • Thermus scotoductus SA-01
  • Uranium(VI) reduction

ASJC Scopus subject areas

  • Environmental Engineering
  • General Chemistry
  • Environmental Chemistry
  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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