TY - JOUR
T1 - Reactive azo compounds as a potential chemotherapy drugs in the treatment of malignant glioblastoma (GBM)
T2 - Experimental and theoretical studies
AU - Udoikono, Akaninyene D.
AU - Louis, Hitler
AU - Eno, Ededet A.
AU - Agwamba, Ernest C.
AU - Unimuke, Tomsmith O.
AU - Igbalagh, Azuaga T.
AU - Edet, Henry O.
AU - Odey, Joseph O.
AU - Adeyinka, Adedapo S.
N1 - Publisher Copyright:
© 2022 The Author(s)
PY - 2022/6
Y1 - 2022/6
N2 - This research work focuses on the synthesis, spectroscopic characterization, DFT studies, and in silico molecular docking of two azo compounds; (E)-6-((4,6-dichloro-1,3,5-triazin-2-yl)amino)-4-hydroxy-3-(phenyldiazenyl)naphthalen-2-yl hydrogen sulfite (compound A) and (E)-6-((4,6-dichloro-1,3,5-triazin-2-yl)amino)-3-((4-formylphenyl)diazenyl)-4-hydroxynaphthalen-2-yl hydrogen sulfite (compound D) to determine their application as chemotherapeutic drug for the treatment of the malignant glioblastoma multiforme (GBM). The experimental and theoretical vibrational wavenumbers of the synthesized compounds were compared and observed to be in good agreement. Density functional theory (DFT) at the B3LYP/6-311++G(d,p) level of theory was further utilized to investigate the frontier molecular orbitals, Fukui reactivity functions, excitation energies, and the natural bond orbital (NBO) analysis for the investigation of the bonding interactions of the studied compounds. The binding affinities of the studied compounds and the standard drug (temozolomide) against four different GBM proteins: 6bft, 6s79, 1Is5, and 1z2b was investigated using in silico molecular docking approach. Compound A displayed the highest relative binding affinities of -8.7 and -8.6 with 6s79 and 1Is5 proteins respectively compared to compound D with the binding affinity of -7.6. Both compounds A and D showed little to no interaction with 1z2b protein but their binding affinities with 6bft, 6s76 and 1Is5 proteins are relatively higher than those of the standard drug. Pharmacological studies also showed that both compounds exhibit good solubility in water resulting in good lipophilicity. With the obtained results, it is safe to say that the compounds and their derivatives could be considered as a potential chemotherapeutic drug for the treatment of glioblastoma or as a precursor for the synthesis of other pharmaceutical products.
AB - This research work focuses on the synthesis, spectroscopic characterization, DFT studies, and in silico molecular docking of two azo compounds; (E)-6-((4,6-dichloro-1,3,5-triazin-2-yl)amino)-4-hydroxy-3-(phenyldiazenyl)naphthalen-2-yl hydrogen sulfite (compound A) and (E)-6-((4,6-dichloro-1,3,5-triazin-2-yl)amino)-3-((4-formylphenyl)diazenyl)-4-hydroxynaphthalen-2-yl hydrogen sulfite (compound D) to determine their application as chemotherapeutic drug for the treatment of the malignant glioblastoma multiforme (GBM). The experimental and theoretical vibrational wavenumbers of the synthesized compounds were compared and observed to be in good agreement. Density functional theory (DFT) at the B3LYP/6-311++G(d,p) level of theory was further utilized to investigate the frontier molecular orbitals, Fukui reactivity functions, excitation energies, and the natural bond orbital (NBO) analysis for the investigation of the bonding interactions of the studied compounds. The binding affinities of the studied compounds and the standard drug (temozolomide) against four different GBM proteins: 6bft, 6s79, 1Is5, and 1z2b was investigated using in silico molecular docking approach. Compound A displayed the highest relative binding affinities of -8.7 and -8.6 with 6s79 and 1Is5 proteins respectively compared to compound D with the binding affinity of -7.6. Both compounds A and D showed little to no interaction with 1z2b protein but their binding affinities with 6bft, 6s76 and 1Is5 proteins are relatively higher than those of the standard drug. Pharmacological studies also showed that both compounds exhibit good solubility in water resulting in good lipophilicity. With the obtained results, it is safe to say that the compounds and their derivatives could be considered as a potential chemotherapeutic drug for the treatment of glioblastoma or as a precursor for the synthesis of other pharmaceutical products.
KW - Azo
KW - DFT
KW - Glioblastoma
KW - Molecular docking
KW - Spectroscopy
KW - Synthesis
UR - http://www.scopus.com/inward/record.url?scp=85131581403&partnerID=8YFLogxK
U2 - 10.1016/j.jpap.2022.100116
DO - 10.1016/j.jpap.2022.100116
M3 - Article
AN - SCOPUS:85131581403
SN - 2666-4690
VL - 10
JO - Journal of Photochemistry and Photobiology
JF - Journal of Photochemistry and Photobiology
M1 - 100116
ER -