Pseudomonas versuta sp. nov., isolated from Antarctic soil

Wah Seng See-Too; Sergio Salazar; Robson Ee; Peter Convey; Kok Gan Chan; Álvaro Peix

doi:10.1016/j.syapm.2017.03.002

Pseudomonas versuta sp. nov., isolated from Antarctic soil

Wah Seng See-Too
, Sergio Salazar
, Robson Ee
, Peter Convey
, Kok Gan Chan
, Álvaro Peix

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

In this study we analysed three bacterial strains coded L10.10^T, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993^T. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4–30 °C, and at pH 4.0–10. The DNA G+C content is 58.2–58.3 mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10^T, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10^T (LMG 29628^T, DSM 101070^T).

Original language	English
Pages (from-to)	191-198
Number of pages	8
Journal	Systematic and Applied Microbiology
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/j.syapm.2017.03.002
Publication status	Published - Jun 2017
Externally published	Yes

Keywords

Antarctic soil
Antarctica
Pseudomonas
Quorum quenching
Quorum sensing
Taxonomy

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Applied Microbiology and Biotechnology

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10.1016/j.syapm.2017.03.002

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title = "Pseudomonas versuta sp. nov., isolated from Antarctic soil",

abstract = "In this study we analysed three bacterial strains coded L10.10T, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7\% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993T. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95\% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86\% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4–30 °C, and at pH 4.0–10. The DNA G+C content is 58.2–58.3 mol \%. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10T, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10T (LMG 29628T, DSM 101070T).",

keywords = "Antarctic soil, Antarctica, Pseudomonas, Quorum quenching, Quorum sensing, Taxonomy",

author = "See-Too, \{Wah Seng\} and Sergio Salazar and Robson Ee and Peter Convey and Chan, \{Kok Gan\} and {\'A}lvaro Peix",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier GmbH",

year = "2017",

month = jun,

doi = "10.1016/j.syapm.2017.03.002",

language = "English",

volume = "40",

pages = "191--198",

journal = "Systematic and Applied Microbiology",

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TY - JOUR

T1 - Pseudomonas versuta sp. nov., isolated from Antarctic soil

AU - See-Too, Wah Seng

AU - Salazar, Sergio

AU - Ee, Robson

AU - Convey, Peter

AU - Chan, Kok Gan

AU - Peix, Álvaro

PY - 2017/6

Y1 - 2017/6

N2 - In this study we analysed three bacterial strains coded L10.10T, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993T. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4–30 °C, and at pH 4.0–10. The DNA G+C content is 58.2–58.3 mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10T, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10T (LMG 29628T, DSM 101070T).

AB - In this study we analysed three bacterial strains coded L10.10T, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993T. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4–30 °C, and at pH 4.0–10. The DNA G+C content is 58.2–58.3 mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10T, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10T (LMG 29628T, DSM 101070T).

KW - Antarctic soil

KW - Antarctica

KW - Pseudomonas

KW - Quorum quenching

KW - Quorum sensing

KW - Taxonomy

UR - https://www.scopus.com/pages/publications/85019056792

U2 - 10.1016/j.syapm.2017.03.002

DO - 10.1016/j.syapm.2017.03.002

M3 - Article

C2 - 28501448

AN - SCOPUS:85019056792

SN - 0723-2020

VL - 40

SP - 191

EP - 198

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 4

ER -

Pseudomonas versuta sp. nov., isolated from Antarctic soil

Abstract

Keywords

ASJC Scopus subject areas

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