Proteomic analysis of Arabidopsis plasma membranes reveals lipopolysaccharide-responsive changes

Nwaxigombe M. Baloyi, Ian A. Dubery, Lizelle A. Piater

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Plant plasma membranes (PMs) contain pattern recognition receptors (PRRs), lately believed to be associated within multicomponent complexes, which perceive microbe-associated molecular pattern (MAMP) molecules like lipopolysaccharides (LPSs) and result in signal transduction events that lead to activated immune defense responses. As such, Arabidopsis thaliana leaves were treated with LPS from Escherichia coli (LPSE.coli) over time, and PM fractions isolated and evaluated using gel-based and subsequent mass spectrometry approaches for identification of LPS-responsive proteins. From the identified protein bands and spots, it is concluded that perception of hexaacylated LPS and resulting signal transduction occurs via PM-associated protein(s), amongst others, receptor-like kinases (RLKs) including G-type lectin S-receptor kinase and BAK1, and mostly likely within specialized perception domains.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume486
Issue number4
DOIs
Publication statusPublished - 13 May 2017

Keywords

  • Lipopolysaccharide
  • Plasma membrane
  • Proteomics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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