Protective effects of parkia biglobosa protein isolate on streptozotocin-induced hepatic damage and oxidative stress in diabetic male rats

Bolajoko Idiat Ogunyinka, Babatunji Emmanuel Oyinloye, Foluso Oluwagbemiga Osunsanmi, Andrew Rowland Opoku, Abidemi Paul Kappo

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

This study sought to investigate the possible protective role of Parkia biglobosa seed protein isolate (PBPi) against streptozotocin-induced hepatic damage and oxidative stress in diabetic male rats. Prior to animal experiments, a HPLC fingerprint of PBPi was recorded. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin (STZ; 60 mg/kg body weight). Diabetic rats were orally treated daily with PBPi (200 or 400 mg/kg body weight) or insulin (5 U/kg, i.p.) for 28 days. The degree of protection was evaluated using biochemical parameters such as malondialdehyde (MDA) levels, serum transaminases (ALT and AST), total protein, total glutathione (Total GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and interleukin-6 (IL-6) activities. Histology of liver sections was also performed. The HPLC fingerprint of PBPi revealed eleven distinct peaks; PBPi at tested doses significantly attenuates STZ-induced elevated levels of serum IL-6, ALT and AST; and hepatic TBARS levels. Hepatic antioxidants (Total GSH, GST, SOD, CAT) as well as total protein were markedly restored in a dose-dependent manner. Histopathological results strongly support the protective role of PBPi. These results suggest PBPi could confer protection by ameliorating hepatic damage and oxidative stress caused by STZ in animal model possibly via its anti-inflammatory and antioxidant properties.

Original languageEnglish
Article number22101654
JournalMolecules
Volume22
Issue number10
DOIs
Publication statusPublished - Oct 2017
Externally publishedYes

Keywords

  • HPLC
  • Hepatic antioxidant
  • Histology
  • Oxidative stress
  • Parkia biglobosa
  • STZ-induced diabetes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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