TY - JOUR
T1 - Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats
AU - Obode, Okukwe
AU - Okafor, Oluwatoyin
AU - Erukainure, Ochuko
AU - Ajayi, Atinuke
AU - Suberu, Yewande
AU - Ogunji, Akinyele
AU - Okporua, Teressa
AU - Oluwole, Oluwatoyin
AU - Ozumba, Augusta
AU - Elemo, Gloria
N1 - Publisher Copyright:
© 2015 by De Gruyter 2015.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Background: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated. Methods: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality. Results: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level. Conclusions: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.
AB - Background: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated. Methods: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality. Results: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level. Conclusions: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.
KW - catalase (CAT)
KW - malondialdehyde (MDA) and reduced glutathione (GSH)
KW - sperm cells
KW - superoxide dismutase (SOD)
UR - https://www.scopus.com/pages/publications/84929579489
U2 - 10.1515/jcim-2014-0032
DO - 10.1515/jcim-2014-0032
M3 - Article
C2 - 25807581
AN - SCOPUS:84929579489
SN - 1553-3840
VL - 12
SP - 137
EP - 142
JO - Journal of Complementary and Integrative Medicine
JF - Journal of Complementary and Integrative Medicine
IS - 2
ER -
