Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

Okukwe Obode; Oluwatoyin Okafor; Ochuko Erukainure; Atinuke Ajayi; Yewande Suberu; Akinyele Ogunji; Teressa Okporua; Oluwatoyin Oluwole; Augusta Ozumba; Gloria Elemo

doi:10.1515/jcim-2014-0032

Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

Okukwe Obode
, Oluwatoyin Okafor
, Ochuko Erukainure
, Atinuke Ajayi
, Yewande Suberu
, Akinyele Ogunji
, Teressa Okporua
, Oluwatoyin Oluwole
, Augusta Ozumba
, Gloria Elemo

Federal Institute of Industrial Research
Albany State University

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated. Methods: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality. Results: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level. Conclusions: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

Original language	English
Pages (from-to)	137-142
Number of pages	6
Journal	Journal of Complementary and Integrative Medicine
Volume	12
Issue number	2
DOIs	https://doi.org/10.1515/jcim-2014-0032
Publication status	Published - 1 Jun 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

catalase (CAT)
malondialdehyde (MDA) and reduced glutathione (GSH)
sperm cells
superoxide dismutase (SOD)

ASJC Scopus subject areas

Complementary and Alternative Medicine

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10.1515/jcim-2014-0032

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title = "Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats",

abstract = "Background: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated. Methods: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality. Results: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level. Conclusions: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.",

keywords = "catalase (CAT), malondialdehyde (MDA) and reduced glutathione (GSH), sperm cells, superoxide dismutase (SOD)",

author = "Okukwe Obode and Oluwatoyin Okafor and Ochuko Erukainure and Atinuke Ajayi and Yewande Suberu and Akinyele Ogunji and Teressa Okporua and Oluwatoyin Oluwole and Augusta Ozumba and Gloria Elemo",

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year = "2015",

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doi = "10.1515/jcim-2014-0032",

language = "English",

volume = "12",

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T1 - Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

AU - Obode, Okukwe

AU - Okafor, Oluwatoyin

AU - Erukainure, Ochuko

AU - Ajayi, Atinuke

AU - Suberu, Yewande

AU - Ogunji, Akinyele

AU - Okporua, Teressa

AU - Oluwole, Oluwatoyin

AU - Ozumba, Augusta

AU - Elemo, Gloria

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Background: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated. Methods: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality. Results: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level. Conclusions: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

AB - Background: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated. Methods: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality. Results: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level. Conclusions: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

KW - catalase (CAT)

KW - malondialdehyde (MDA) and reduced glutathione (GSH)

KW - sperm cells

KW - superoxide dismutase (SOD)

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DO - 10.1515/jcim-2014-0032

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AN - SCOPUS:84929579489

SN - 1553-3840

VL - 12

SP - 137

EP - 142

JO - Journal of Complementary and Integrative Medicine

JF - Journal of Complementary and Integrative Medicine

IS - 2

ER -

Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

Abstract

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Keywords

ASJC Scopus subject areas

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