TY - JOUR
T1 - Prevalence and distribution of antimicrobial resistance determinants of Escherichia coli isolates obtained from meat in South Africa
AU - Jaja, Ishmael Festus
AU - Oguttu, James
AU - Jaja, Chinwe Juliana Iwu
AU - Green, Ezekiel
N1 - Publisher Copyright:
© 2020 Jaja et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2020/5
Y1 - 2020/5
N2 - Objective This study aimed to characterise antibiotics resistance of Escherichia coli isolates from the formal meat sector (FMS) and informal meat sectors (INMS). Method A total of 162 and 102 E. coli isolates from the FMS, and INMS respectively were isolated by standard culture-based, and biochemical reactions. The isolates were further confirmed by polymerase chain reaction (PCR). The disc diffusion method was used to screen for antimicrobial susceptibility against 19 different antibiotics. The presence of class 1-2 integrons in each E. coli isolates was assessed using 30-CS and 50-CS regions specific primers. Result Among the 19 antimicrobials, resistance to tetracyclines, aminoglycosides, cephalosporins, and nitrofurans were found to be more frequent than carbapenems and chloramphenicol. The number of multi-drug resistance ranged from three to ten antimicrobials. The resistant determinants with the highest prevalence in the FMS and INMS were; [aminoglycosides: AadA (40.6%; 31.9%), and strA (6.5%; 9.4%)], [β-lactams: AmpC (20%; 45%),], [Chloramphenicol: CatI (1.7%; 1.7%), and [tetracyclines: TetB (11.5%; 24%),], and [sulfonamides: Sul1 (22.2%; 26.7%),]. Conclusion Higher phenotypic resistance to cephalosporins and carbapenems were found in the FMS than in INMS. The multiple antibiotic resistance (MAR) indexes for FMS and INMS ranged from 0.2-0.5. The results reveal a high prevalence of multidrug-resistant E. coli isolates and resistance determinants, suggesting that consumers and handlers of such meat are at risk of contracting antibiotic-resistant E. coli-related foodborne disease.
AB - Objective This study aimed to characterise antibiotics resistance of Escherichia coli isolates from the formal meat sector (FMS) and informal meat sectors (INMS). Method A total of 162 and 102 E. coli isolates from the FMS, and INMS respectively were isolated by standard culture-based, and biochemical reactions. The isolates were further confirmed by polymerase chain reaction (PCR). The disc diffusion method was used to screen for antimicrobial susceptibility against 19 different antibiotics. The presence of class 1-2 integrons in each E. coli isolates was assessed using 30-CS and 50-CS regions specific primers. Result Among the 19 antimicrobials, resistance to tetracyclines, aminoglycosides, cephalosporins, and nitrofurans were found to be more frequent than carbapenems and chloramphenicol. The number of multi-drug resistance ranged from three to ten antimicrobials. The resistant determinants with the highest prevalence in the FMS and INMS were; [aminoglycosides: AadA (40.6%; 31.9%), and strA (6.5%; 9.4%)], [β-lactams: AmpC (20%; 45%),], [Chloramphenicol: CatI (1.7%; 1.7%), and [tetracyclines: TetB (11.5%; 24%),], and [sulfonamides: Sul1 (22.2%; 26.7%),]. Conclusion Higher phenotypic resistance to cephalosporins and carbapenems were found in the FMS than in INMS. The multiple antibiotic resistance (MAR) indexes for FMS and INMS ranged from 0.2-0.5. The results reveal a high prevalence of multidrug-resistant E. coli isolates and resistance determinants, suggesting that consumers and handlers of such meat are at risk of contracting antibiotic-resistant E. coli-related foodborne disease.
UR - http://www.scopus.com/inward/record.url?scp=85085539465&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0216914
DO - 10.1371/journal.pone.0216914
M3 - Article
C2 - 32453796
AN - SCOPUS:85085539465
SN - 1932-6203
VL - 15
JO - PLoS ONE
JF - PLoS ONE
IS - 5
M1 - e0216914
ER -