Potentiation of antimicrobial photodynamic inactivation mediated by a cationic fullerene by added iodide: In vitro and in vivo studies

Yunsong Zhang, Tianhong Dai, Min Wang, Daniela Vecchio, Long Y. Chiang, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)

Abstract

Background: Antimicrobial photodynamic inactivation with fullerenes bearing cationic charges may overcome resistant microbes. Methods & results: We synthesized C60-fullerene (LC16) bearing decaquaternary chain and deca-tertiary-amino groups that facilitates electron-transfer reactions via the photoexcited fullerene. Addition of the harmless salt, potassium iodide (10 mM) potentiated the ultraviolet A (UVA) or white light-mediated killing of Gram-negative bacteria Acinetobacter baumannii, Gram-positive methicillin-resistant Staphylococcus aureus and fungal yeast Candida albicans by 1-2+ logs. Mouse model infected with bioluminescent Acinetobacter baumannii gave increased loss of bioluminescence when iodide (10 mM) was combined with LC16 and UVA/white light. Conclusion: The mechanism may involve photoinduced electron reduction of 1(C60>)∗ or 3(C60>)∗ by iodide producing I· or I2 followed by subsequent intermolecular electron-transfer events of (C60>)-· to produce reactive radicals.

Original languageEnglish
Pages (from-to)603-614
Number of pages12
JournalNanomedicine
Volume10
Issue number4
DOIs
Publication statusPublished - 1 Mar 2015
Externally publishedYes

Keywords

  • antimicrobial photodynamic therapy
  • bioluminescent bacteria
  • decacationic fullerene
  • decatertiary-amine chain
  • in vivo infection model
  • iodide potentiation

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • General Materials Science

Fingerprint

Dive into the research topics of 'Potentiation of antimicrobial photodynamic inactivation mediated by a cationic fullerene by added iodide: In vitro and in vivo studies'. Together they form a unique fingerprint.

Cite this