Phototherapy promotes cell migration in the presence of hydroxyurea

I. L. Zungu, A. B. Mbene, D. H. Hawkins Evans, N. N. Houreld, H. Abrahamse

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Phototherapy has been shown to cause an increase in cell proliferation and migration. This study focused on viability (trypan blue), proliferation [sodium 3-(1-(phenylaminocarbonyl)-3,4-tetrazolium)-bis(4-methoxy-6-nitro)-benzene sulphonic acid hydrate (XTT) and adenosine triphosphate (ATP)] and migration of WS1 cells following irradiation in the presence of hydroxyurea (HU), which is an inhibitor of proliferation. Wounded cells were irradiated on days 1 and 4 with a fluence of 5 J/cm2 with a helium-neon (He-Ne) laser at 632.8 nm. After a repair time of 24 h, cellular responses were assessed. Wounded irradiated cells without HU showed an increase in cell viability and proliferation, which was confirmed by complete wound closure by day 4. Although wounded irradiated cells treated with 5 mM HU showed incomplete wound closure, these cells showed increased migration compared with that of control cells. This study showed that laser irradiation using an He-Ne laser with a fluence of 5 J/cm2 stimulates cell viability. The HU results confirmed that laser irradiation promotes cell migration and proliferation.

Original languageEnglish
Pages (from-to)144-150
Number of pages7
JournalLasers in Medical Science
Issue number2
Publication statusPublished - Mar 2009


  • Fibroblasts
  • Hydroxyurea
  • Migration
  • Phototherapy
  • Proliferation
  • Viability

ASJC Scopus subject areas

  • Surgery
  • Dermatology


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