Photonic real-time monitoring of bacterial reduction in root canals by genetically engineered bacteria after chemomechanical endodontic therapy

Aguinaldo Silva Garcez, Silvia Cristina Nunez, José Luis Lage-Marques, Michael R. Hamblin, Martha Simões Ribeiro

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Microbial infection plays an important role in the development of pulp necrosis and formation of periapical lesions. In vitro and in vivo research in this field, traditionally microbiological culture methods using paper point sampling and quantitative culture, faces difficultiesin completely removing bacteria from the root canal system and analyzing sequential procedures. This study employed genetically engineered bioluminescent bacteria and a light-sensitive imaging system to allow real-time visualization of the infection. Ten extracted teeth incubated with P. aeruginosa were treated by mechanical instrumentation with K-files (#30 K-file, #35 K-file and #40 K-file) and chemical irrigation with sodium hypochlorite and hydrogen peroxide. Irrigation alone reduced the contamination in 18%; the first chemomechanical sequence (instrumentation with a #30 K-file + irrigation) provided 41% of reduction; the second sequence (#35 Kfile + irrigation) achieved 62%; and the complete therapy (#30 K-file + #35 K-file + #40 K-file + irrigation) achieved 93% of bacterial reduction. These results suggest that the endodontic treatment is dependent on the association of a chemical and mechanical approaches and that root canal enlargement improves bacterial reduction probably because the irrigation has more access to the apical third.

Original languageEnglish
Pages (from-to)202-207
Number of pages6
JournalBrazilian Dental Journal
Volume18
Issue number3
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Bacteria
  • Chemomechanical endodontic therapy
  • Root canals

ASJC Scopus subject areas

  • General Dentistry

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