Photodynamic therapy with hyperbranched poly(ether-ester) chlorin(e6) nanoparticles on human tongue carcinoma CAL-27 cells

Pingping Li, Guoyu Zhou, Xinyuan Zhu, Guolin Li, Peng Yan, Linyue Shen, Qin Xu, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Background: Hyperbranched polymers represent a new class of drug-delivery vehicle that can be used to prepare nanoparticles with uniform size distribution. Methods: In this study we prepared covalent conjugates between the photosensitizer chlorin(e6) and hyperbranched poly(ether-ester), HPEE. HPEE-ce6 nanoparticles were synthesized by carbodiimide-mediated reaction between HPEE and ce6, and characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), and transmission electron microscopy (TEM). The uptake and phototoxicity of HPEE-ce6 nanoparticles towards human oral tongue cancer CAL-27 cells was detected by confocal laser scanning microscopy (CLSM) and MTT assay, respectively. Results: The absorption peak of HPEE-ce6 nanoparticles was red-shifted 12-nm compared with ce6, and TEM showed uniform nanoparticles with a diameter of 50-nm. HPEE-ce6 nanoparticles were taken up by CAL-27 cells after 4h incubation and localized in the cytoplasm. The MTT assay showed a significantly (P<0.05) higher phototoxicity compared to free ce6 after 12J/cm 2 of 660-nm laser illumination. Conclusions: This is the first time to our knowledge that hyperbranched polymers have been used in PDT drug delivery.

Original languageEnglish
Pages (from-to)76-82
Number of pages7
JournalPhotodiagnosis and Photodynamic Therapy
Volume9
Issue number1
DOIs
Publication statusPublished - Mar 2012
Externally publishedYes

Keywords

  • Chlorin(e6)
  • Drug delivery system (DDS)
  • Hyperbranched poly(ether-ester) (HPEE)
  • Nanoparticles
  • Photodynamic therapy (PDT)
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Biophysics
  • Oncology
  • Dermatology
  • Pharmacology (medical)

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