TY - JOUR
T1 - Photodynamic therapy of murine mastocytoma induces specific immune responses against the cancer/testis antigen P1A
AU - Mroz, Pawel
AU - Vatansever, Fatma
AU - Muchowicz, Angelika
AU - Hamblin, Michael R.
PY - 2013/11/1
Y1 - 2013/11/1
N2 - Photodynamic therapy (PDT) involves the intravenous administration of photosensitizers followed by illumination of the tumor with visible light, leading to local production of reactive oxygen species that cause vascular shutdown and tumor cell death. Antitumor immunity is stimulated after PDT because of the acute inflammatory response that involves activation of the innate immune system, leading to stimulation of adaptive immunity. We carried out PDT using benzoporphyrin derivative and 690-nm light after 15 minutes, in DBA/2 mice bearing either the mastocytoma, P815, which expresses the naturally occurring cancer/testis antigen P1A, or the corresponding tumor P1.204 that lacks P1A expression. Tumor cures, significantly higher survival, and rejection of tumor rechallenge were obtained with P815, which were not seen with P1.204 or seen with P815 growing in nude mice. Both CD4 and CD8 T cells had higher levels of intracellular cytokines when isolated from mice receiving PDT of P815 tumors than P1.204 tumors and CD8 T cells from P815-cured mice recognized the peptide epitope of the P1A antigen (LPYLGWLVF) using pentamer staining. Taken together, these findings show thatPDTcan induce a potent antigen- and epitope-specific immune response against a naturally occurring mouse tumor antigen.
AB - Photodynamic therapy (PDT) involves the intravenous administration of photosensitizers followed by illumination of the tumor with visible light, leading to local production of reactive oxygen species that cause vascular shutdown and tumor cell death. Antitumor immunity is stimulated after PDT because of the acute inflammatory response that involves activation of the innate immune system, leading to stimulation of adaptive immunity. We carried out PDT using benzoporphyrin derivative and 690-nm light after 15 minutes, in DBA/2 mice bearing either the mastocytoma, P815, which expresses the naturally occurring cancer/testis antigen P1A, or the corresponding tumor P1.204 that lacks P1A expression. Tumor cures, significantly higher survival, and rejection of tumor rechallenge were obtained with P815, which were not seen with P1.204 or seen with P815 growing in nude mice. Both CD4 and CD8 T cells had higher levels of intracellular cytokines when isolated from mice receiving PDT of P815 tumors than P1.204 tumors and CD8 T cells from P815-cured mice recognized the peptide epitope of the P1A antigen (LPYLGWLVF) using pentamer staining. Taken together, these findings show thatPDTcan induce a potent antigen- and epitope-specific immune response against a naturally occurring mouse tumor antigen.
UR - http://www.scopus.com/inward/record.url?scp=84887206006&partnerID=8YFLogxK
U2 - 10.1158/0008-5472.CAN-11-2572
DO - 10.1158/0008-5472.CAN-11-2572
M3 - Article
C2 - 24072749
AN - SCOPUS:84887206006
SN - 0008-5472
VL - 73
SP - 6462
EP - 6470
JO - Cancer Research
JF - Cancer Research
IS - 21
ER -