TY - GEN
T1 - Photodynamic therapy improves the ultraviolet-irradiated hairless mice skin
AU - Jorge, Ana Elisa S.
AU - Hamblin, Michael R.
AU - Parizotto, Nivaldo A.
AU - Kurachi, Cristina
AU - Bagnato, Vanderlei S.
PY - 2014
Y1 - 2014
N2 - Chronic exposure to ultraviolet (UV) sunlight causes premature skin aging. In light of this fact, photodynamic therapy (PDT) is an emerging modality for treating cancer and other skin conditions, however its response on photoaged skin has not been fully illustrated by means of histopathology. For this reason, the aim of this study was analyze whether PDT can play a role on a mouse model of photoaging. Hence, SKH-1 hairless mice were randomly allocated in two groups, UV and UV/PDT. The mice were daily exposed to an UV light source (280-400 nm: peak at 350 nm) for 8 weeks followed by a single PDT session using 20% 5-aminolevulinic acid (ALA) topically. After the proper photosensitizer accumulation within the tissue, a non-coherent red (635 nm) light was performed and, after 14 days, skin samples were excised and processed for light microscopy, and their sections were stained with hematoxylin-eosin (HE) and Massona's Trichrome. As a result, we observed a substantial epidermal thickening and an improvement in dermal collagen density by deposition of new collagen fibers on UV/PDT group. These findings strongly indicate epidermal and dermal restoration, and consequently skin restoration. In conclusion, this study provides suitable evidences that PDT improves the UV-irradiated hairless mice skin, supporting this technique as an efficient treatment for photoaged skin.
AB - Chronic exposure to ultraviolet (UV) sunlight causes premature skin aging. In light of this fact, photodynamic therapy (PDT) is an emerging modality for treating cancer and other skin conditions, however its response on photoaged skin has not been fully illustrated by means of histopathology. For this reason, the aim of this study was analyze whether PDT can play a role on a mouse model of photoaging. Hence, SKH-1 hairless mice were randomly allocated in two groups, UV and UV/PDT. The mice were daily exposed to an UV light source (280-400 nm: peak at 350 nm) for 8 weeks followed by a single PDT session using 20% 5-aminolevulinic acid (ALA) topically. After the proper photosensitizer accumulation within the tissue, a non-coherent red (635 nm) light was performed and, after 14 days, skin samples were excised and processed for light microscopy, and their sections were stained with hematoxylin-eosin (HE) and Massona's Trichrome. As a result, we observed a substantial epidermal thickening and an improvement in dermal collagen density by deposition of new collagen fibers on UV/PDT group. These findings strongly indicate epidermal and dermal restoration, and consequently skin restoration. In conclusion, this study provides suitable evidences that PDT improves the UV-irradiated hairless mice skin, supporting this technique as an efficient treatment for photoaged skin.
KW - Dermal collagen density
KW - Epidermal thickening
KW - Hairless mice
KW - Histopathology
KW - Photodynamic therapy
KW - UV-irradiated skin
UR - http://www.scopus.com/inward/record.url?scp=84897556806&partnerID=8YFLogxK
U2 - 10.1117/12.2040297
DO - 10.1117/12.2040297
M3 - Conference contribution
AN - SCOPUS:84897556806
SN - 9780819498397
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photonic Therapeutics and Diagnostics X
PB - SPIE
T2 - Photonic Therapeutics and Diagnostics X
Y2 - 1 February 2014 through 2 February 2014
ER -
