Photodynamic therapy for methicillin-resistant Staphylococcus aureus infection in a mouse skin abrasion model

Tianhong Dai, George P. Tegos, Timur Zhiyentayev, Eleftherios Mylonakis, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

193 Citations (Scopus)

Abstract

Background and Objective: Methicillin-resistant Staphylococcus aureus (MRSA) skin infections are now knownto be acommonand important problem in the Unites States. The objective of this study was to investigate the efficacy of photodynamic therapy (PDT) for the treatment of MRSA infection in skin abrasion wounds using a mouse model. Study Design/Materials and Methods: A mouse model of skin abrasion wound infected with MRSA was developed. Bioluminescent strain of MRSA, a derivative of ATCC 33591, was used to allow the real-time monitoring of the extent of infection in mouse wounds. PDT was performed with the combination of a polyethylenimine (PEI)-ce6 photosensitizer (PS) and non-coherent red light. In vivo fluorescence imaging was carried out to evaluate the effect of photobleaching of PS during PDT. Results: In vivo fluorescence imaging of conjugate PEI-ce6 applied in mice indicated the photobleaching effect of the PS during PDT. PDT induced on average 2.7 log10 of inactivation of MRSA as judged by loss of bioluminescence in mouse skin abrasion wounds and accelerated the wound healing on average by 8.6 days in comparison to the untreated infected wounds. Photobleaching of PS in the wound was overcome by adding the PS solution in aliquots. Conclusion: PDT may represent an alternative approach for the treatment of MRSA skin infections.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalLasers in Surgery and Medicine
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

Keywords

  • Bioluminescence imaging
  • Fluorescence imaging
  • MRSA
  • Mouse model
  • Photodynamic therapy
  • Skin abrasion
  • Wound infection

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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