Photodynamic therapy can induce a protective innate immune response against murine bacterial Arthritis via Neutrophil accumulation

Masamitsu Tanaka, Pawel Mroz, Tianhong Dai, Liyi Huang, Yuji Morimoto, Manabu Kinoshita, Yasuo Yoshihara, Koichi Nemoto, Nariyoshi Shinomiya, Suhji Seki, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)


Background: Local microbial infections induced by multiple-drug-resistant bacteria in the orthopedic field can be intractable, therefore development of new therapeutic modalities is needed. Photodynamic therapy (PDT) is a promising alternative modality to antibiotics for intractable microbial infections, and we recently reported that PDT has the potential to accumulate neutrophils into the infected site which leads to resolution of the infection. PDT for cancer has long been known to be able to stimulate the innate and adaptive arms of the immune system. Methodology/Principal Findings: In the present study, a murine methicillin-resistant Staphylococcus aureus (MRSA) arthritis model using bioluminescent MRSA and polystyrene microparticles was established, and both the therapeutic (Th-PDT) and preventive (Pre-PDT) effects of PDT using methylene blue as photosensitizer were examined. Although Th-PDT could not demonstrate direct bacterial killing, neutrophils were accumulated into the infectious joint space after PDT and MRSA arthritis was reduced. With the preconditioning Pre-PDT regimen, neutrophils were quickly accumulated into the joint immediately after bacterial inoculation and bacterial growth was suppressed and the establishment of infection was inhibited. Conclusions/Significance: This is the first demonstration of a protective innate immune response against a bacterial pathogen produced by PDT.

Original languageEnglish
Article numbere39823
JournalPLoS ONE
Issue number6
Publication statusPublished - 26 Jun 2012
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • Multidisciplinary


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