Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

Ivan Mfouo-Tynga; Nicolette Nadene Houreld; Heidi Abrahamse

doi:10.1117/12.2184043

Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

Laser Research Centre

University of Johannesburg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.

Original language	English
Title of host publication	European Conference on Biomedical Optics, ECBO 2015
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Print)	9781628417012
DOIs	https://doi.org/10.1117/12.2184043
Publication status	Published - 2015
Event	European Conference on Biomedical Optics, ECBO 2015 - Munich, Germany Duration: 21 Jun 2015 → 25 Jun 2015

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	European Conference on Biomedical Optics, ECBO 2015
Country/Territory	Germany
City	Munich
Period	21/06/15 → 25/06/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Breast cancer
Cell damage
Gold nanoparticles
Light activation
Photodynamic cancer therapy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Mechanics of Materials

Access to Document

10.1117/12.2184043

Cite this

@inproceedings{c184be29170e402fbe528023ec358f2b,

title = "Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro",

abstract = "Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.",

keywords = "Breast cancer, Cell damage, Gold nanoparticles, Light activation, Photodynamic cancer therapy",

author = "Ivan Mfouo-Tynga and Houreld, \{Nicolette Nadene\} and Heidi Abrahamse",

note = "Publisher Copyright: {\textcopyright} 2015 SPIE-OSA.; European Conference on Biomedical Optics, ECBO 2015 ; Conference date: 21-06-2015 Through 25-06-2015",

year = "2015",

doi = "10.1117/12.2184043",

language = "English",

isbn = "9781628417012",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "European Conference on Biomedical Optics, ECBO 2015",

}

Mfouo-Tynga, I, Houreld, NN & Abrahamse, H 2015, Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro. in European Conference on Biomedical Optics, ECBO 2015. Optics InfoBase Conference Papers, Optica Publishing Group (formerly OSA), European Conference on Biomedical Optics, ECBO 2015, Munich, Germany, 21/06/15. https://doi.org/10.1117/12.2184043

Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro. / Mfouo-Tynga, Ivan; Houreld, Nicolette Nadene ; Abrahamse, Heidi.
European Conference on Biomedical Optics, ECBO 2015. Optica Publishing Group (formerly OSA), 2015. (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

AU - Mfouo-Tynga, Ivan

AU - Houreld, Nicolette Nadene

AU - Abrahamse, Heidi

PY - 2015

Y1 - 2015

N2 - Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.

AB - Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.

KW - Breast cancer

KW - Cell damage

KW - Gold nanoparticles

KW - Light activation

KW - Photodynamic cancer therapy

UR - https://www.scopus.com/pages/publications/85165739145

U2 - 10.1117/12.2184043

DO - 10.1117/12.2184043

M3 - Conference contribution

AN - SCOPUS:85165739145

SN - 9781628417012

T3 - Optics InfoBase Conference Papers

BT - European Conference on Biomedical Optics, ECBO 2015

PB - Optica Publishing Group (formerly OSA)

T2 - European Conference on Biomedical Optics, ECBO 2015

Y2 - 21 June 2015 through 25 June 2015

ER -

Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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