Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

Ivan Mfouo-Tynga; Nicolette N. Houreld; Heidi Abrahamse

doi:10.1117/12.2184043

Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

Laser Research Centre

University of Johannesburg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.

Original language	English
Title of host publication	Medical Laser Applications and Laser-Tissue Interactions VII
Editors	Ronald Sroka, Lothar D. Lilge
Publisher	SPIE
ISBN (Electronic)	9781628417074
DOIs	https://doi.org/10.1117/12.2184043
Publication status	Published - 2015
Event	Medical Laser Applications and Laser Tissue Interactions VII - Munich, Germany Duration: 21 Jun 2015 → 23 Jun 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9542
ISSN (Print)	1605-7422

Conference

Conference	Medical Laser Applications and Laser Tissue Interactions VII
Country/Territory	Germany
City	Munich
Period	21/06/15 → 23/06/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Breast cancer
Cell damage
Gold nanoparticles
Light activation
Photodynamic cancer therapy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology, Nuclear Medicine and Imaging

Access to Document

10.1117/12.2184043

Cite this

Mfouo-Tynga, I., Houreld, N. N., & Abrahamse, H. (2015). Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro. In R. Sroka, & L. D. Lilge (Eds.), Medical Laser Applications and Laser-Tissue Interactions VII Article 954204 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9542). SPIE. https://doi.org/10.1117/12.2184043

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title = "Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro",

abstract = "Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.",

keywords = "Breast cancer, Cell damage, Gold nanoparticles, Light activation, Photodynamic cancer therapy",

author = "Ivan Mfouo-Tynga and Houreld, \{Nicolette N.\} and Heidi Abrahamse",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE-OSA.; Medical Laser Applications and Laser Tissue Interactions VII ; Conference date: 21-06-2015 Through 23-06-2015",

year = "2015",

doi = "10.1117/12.2184043",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Mfouo-Tynga, I, Houreld, NN & Abrahamse, H 2015, Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro. in R Sroka & LD Lilge (eds), Medical Laser Applications and Laser-Tissue Interactions VII., 954204, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9542, SPIE, Medical Laser Applications and Laser Tissue Interactions VII, Munich, Germany, 21/06/15. https://doi.org/10.1117/12.2184043

Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro. / Mfouo-Tynga, Ivan; Houreld, Nicolette N.; Abrahamse, Heidi.
Medical Laser Applications and Laser-Tissue Interactions VII. ed. / Ronald Sroka; Lothar D. Lilge. SPIE, 2015. 954204 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9542).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

AU - Mfouo-Tynga, Ivan

AU - Houreld, Nicolette N.

AU - Abrahamse, Heidi

PY - 2015

Y1 - 2015

N2 - Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.

AB - Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.

KW - Breast cancer

KW - Cell damage

KW - Gold nanoparticles

KW - Light activation

KW - Photodynamic cancer therapy

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DO - 10.1117/12.2184043

M3 - Conference contribution

AN - SCOPUS:84939549444

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Medical Laser Applications and Laser-Tissue Interactions VII

A2 - Sroka, Ronald

A2 - Lilge, Lothar D.

PB - SPIE

T2 - Medical Laser Applications and Laser Tissue Interactions VII

Y2 - 21 June 2015 through 23 June 2015

ER -

Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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