TY - GEN
T1 - Photodynamic effects of gold nanoparticles in a breast cancer cell line (MCF-7) in vitro
AU - Mfouo-Tynga, Ivan
AU - Houreld, Nicolette Nadene
AU - Abrahamse, Heidi
N1 - Publisher Copyright:
© 2015 SPIE-OSA.
PY - 2015
Y1 - 2015
N2 - Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.
AB - Photodynamic cancer therapy (PDT) had already proved its effectiveness in vitro but the search for more effective photosensitizers is encouraged and ongoing. Gold nanoparticles (AuNPs), have been shown to be good drug delivery agents, but in this work AuNPs were investigated as photodynamic agents for PDT. AuNPs were synthesized and characterized by means UV-Vis spectroscopy and transmission electron microscopy (TEM). Photodynamic effects of AuNPs in MCF-7 cells were evaluated using trypan blue, adenosine triphosphate (ATP) luminescence and lactate dehydrogenase (LDH) membrane integrity for cell viability, proliferation and cytotoxicity, respectively. AuNPs had a peak absorption at 540 nm, spherical in shape and were successfully taken up by MCF-7 cells. As a result of light activation or not, cell damage was observed and AuNPs modified into dendrimer-entrapped gold nanoparticles (AuDENPs), which only yielded effects in a light dependent manner. AuNPs is not a suitable photodynamic agent but its modified AuDENPs can be essential in improving the efficiency of PDT.
KW - Breast cancer
KW - Cell damage
KW - Gold nanoparticles
KW - Light activation
KW - Photodynamic cancer therapy
UR - http://www.scopus.com/inward/record.url?scp=85165739145&partnerID=8YFLogxK
U2 - 10.1117/12.2184043
DO - 10.1117/12.2184043
M3 - Conference contribution
AN - SCOPUS:85165739145
SN - 9781628417012
T3 - Optics InfoBase Conference Papers
BT - European Conference on Biomedical Optics, ECBO 2015
PB - Optica Publishing Group (formerly OSA)
T2 - European Conference on Biomedical Optics, ECBO 2015
Y2 - 21 June 2015 through 25 June 2015
ER -
