Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells

S. W. Jere; N. N. Houreld; H. Abrahamse

doi:10.1117/12.2539096

Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells

Laser Research Centre

University of Johannesburg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

In the presence of diabetes mellitus (DM), the wound healing process is interrupted, and chronic wounds develop. DM affects the wound healing response and leads to early apoptosis and reduced number/function of fibroblast cells. In this study we investigated the hypothesis that photobiomodulation (PBM) initiates healing through reduced apoptosis and increased cell survival in diabetic wounded fibroblast (WS1) cells. Cells were irradiated using a diode laser at a wavelength of 660 nm, power output of 100 mW/cm² and a fluence of 5 J/cm². Non-irradiated cells were used as controls. Irradiated cells were incubated for 48 h, after which assays for cell viability (Trypan blue exclusion assay), proliferation (5-Bromo-2′-deoxyuridine-BrdU), apoptosis (Annexin V/PI) and the expression of B-Cell Lymphoma 2- Bcl2 (ELISA) were done. PBM at a wavelength of 660 nm, power output of 100 mW/cm² and a fluence of 5 J/cm² significantly increased cell survival through reduced apoptosis in diabetic wounded fibroblast cells. The PBM effects observed in this study are critical in the wound healing process.

Original language	English
Title of host publication	Mechanisms of Photobiomodulation Therapy XV
Editors	Michael R. Hamblin, James D. Carroll, Praveen Arany
Publisher	SPIE
ISBN (Electronic)	9781510632059
DOIs	https://doi.org/10.1117/12.2539096
Publication status	Published - 2020
Event	Mechanisms of Photobiomodulation Therapy XV 2020 - San Francisco, United States Duration: 1 Feb 2020 → …

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11221
ISSN (Print)	1605-7422

Conference

Conference	Mechanisms of Photobiomodulation Therapy XV 2020
Country/Territory	United States
City	San Francisco
Period	1/02/20 → …

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Cell survival
Chronic wounds
Diabetes mellitus
Wound healing

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology, Nuclear Medicine and Imaging

Access to Document

10.1117/12.2539096

Cite this

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title = "Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells",

abstract = "In the presence of diabetes mellitus (DM), the wound healing process is interrupted, and chronic wounds develop. DM affects the wound healing response and leads to early apoptosis and reduced number/function of fibroblast cells. In this study we investigated the hypothesis that photobiomodulation (PBM) initiates healing through reduced apoptosis and increased cell survival in diabetic wounded fibroblast (WS1) cells. Cells were irradiated using a diode laser at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2. Non-irradiated cells were used as controls. Irradiated cells were incubated for 48 h, after which assays for cell viability (Trypan blue exclusion assay), proliferation (5-Bromo-2′-deoxyuridine-BrdU), apoptosis (Annexin V/PI) and the expression of B-Cell Lymphoma 2- Bcl2 (ELISA) were done. PBM at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2 significantly increased cell survival through reduced apoptosis in diabetic wounded fibroblast cells. The PBM effects observed in this study are critical in the wound healing process.",

keywords = "Apoptosis, Cell survival, Chronic wounds, Diabetes mellitus, Wound healing",

author = "Jere, \{S. W.\} and Houreld, \{N. N.\} and H. Abrahamse",

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language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Jere, SW, Houreld, NN & Abrahamse, H 2020, Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells. in MR Hamblin, JD Carroll & P Arany (eds), Mechanisms of Photobiomodulation Therapy XV., 1122103, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11221, SPIE, Mechanisms of Photobiomodulation Therapy XV 2020, San Francisco, United States, 1/02/20. https://doi.org/10.1117/12.2539096

Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells. / Jere, S. W.; Houreld, N. N.; Abrahamse, H.
Mechanisms of Photobiomodulation Therapy XV. ed. / Michael R. Hamblin; James D. Carroll; Praveen Arany. SPIE, 2020. 1122103 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11221).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

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AU - Jere, S. W.

AU - Houreld, N. N.

AU - Abrahamse, H.

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2020

Y1 - 2020

N2 - In the presence of diabetes mellitus (DM), the wound healing process is interrupted, and chronic wounds develop. DM affects the wound healing response and leads to early apoptosis and reduced number/function of fibroblast cells. In this study we investigated the hypothesis that photobiomodulation (PBM) initiates healing through reduced apoptosis and increased cell survival in diabetic wounded fibroblast (WS1) cells. Cells were irradiated using a diode laser at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2. Non-irradiated cells were used as controls. Irradiated cells were incubated for 48 h, after which assays for cell viability (Trypan blue exclusion assay), proliferation (5-Bromo-2′-deoxyuridine-BrdU), apoptosis (Annexin V/PI) and the expression of B-Cell Lymphoma 2- Bcl2 (ELISA) were done. PBM at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2 significantly increased cell survival through reduced apoptosis in diabetic wounded fibroblast cells. The PBM effects observed in this study are critical in the wound healing process.

AB - In the presence of diabetes mellitus (DM), the wound healing process is interrupted, and chronic wounds develop. DM affects the wound healing response and leads to early apoptosis and reduced number/function of fibroblast cells. In this study we investigated the hypothesis that photobiomodulation (PBM) initiates healing through reduced apoptosis and increased cell survival in diabetic wounded fibroblast (WS1) cells. Cells were irradiated using a diode laser at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2. Non-irradiated cells were used as controls. Irradiated cells were incubated for 48 h, after which assays for cell viability (Trypan blue exclusion assay), proliferation (5-Bromo-2′-deoxyuridine-BrdU), apoptosis (Annexin V/PI) and the expression of B-Cell Lymphoma 2- Bcl2 (ELISA) were done. PBM at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2 significantly increased cell survival through reduced apoptosis in diabetic wounded fibroblast cells. The PBM effects observed in this study are critical in the wound healing process.

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BT - Mechanisms of Photobiomodulation Therapy XV

A2 - Hamblin, Michael R.

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PB - SPIE

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Y2 - 1 February 2020

ER -

Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

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