Phenylalanine-Arginine-b-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa

Lixia He; Shanlin Yang; Weijun Xuan; Xiumei Zhen; Qi Qi; Yongqi Qi; Qingxiang Li; Meixia Du; Michael R. Hamblin; Liyi Huang

doi:10.1089/photob.2023.0005

Phenylalanine-Arginine-b-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa

Lixia He, Shanlin Yang, Weijun Xuan, Xiumei Zhen, Qi Qi, Yongqi Qi, Qingxiang Li, Meixia Du, Michael R. Hamblin, Liyi Huang

Laser Research Centre

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-b-naphthylamide (PAbN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5–240 min), and then, 10 J/cm² red light was irradiated. The efflux pump inhibitor (EPI) PAbN (10–100 lg/mL) was combined with MB (1–20 lM) in different sequences (PAbN-first, PAbN+MB, PAbN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 lM plus 10 J/cm², the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAbN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAbN-first had the best killing effect, whereas PAbN-after had the worst killing effect. The killing effect increased with PAbN concentration and at 100 lg/mL reached 5.1 logs. Conclusions: The EPI PAbN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.

Original language	English
Pages (from-to)	569-575
Number of pages	7
Journal	Photobiomodulation, Photomedicine, and Laser Surgery
Volume	41
Issue number	10
DOIs	https://doi.org/10.1089/photob.2023.0005
Publication status	Published - 1 Oct 2023

Keywords

Pseudomonas aeruginosa
antimicrobial photodynamic inactivation
efflux pump inhibitor
methylene blue
phenylalanine-arginine-b-naphthylamide
resistance-nodulation-division family efflux pump

ASJC Scopus subject areas

Radiology, Nuclear Medicine and Imaging
Biomedical Engineering

Access to Document

10.1089/photob.2023.0005

Cite this

@article{a8e21ed523c84781927fb4eea77f5453,

title = "Phenylalanine-Arginine-b-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa",

abstract = "Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-b-naphthylamide (PAbN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5–240 min), and then, 10 J/cm2 red light was irradiated. The efflux pump inhibitor (EPI) PAbN (10–100 lg/mL) was combined with MB (1–20 lM) in different sequences (PAbN-first, PAbN+MB, PAbN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 lM plus 10 J/cm2, the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAbN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAbN-first had the best killing effect, whereas PAbN-after had the worst killing effect. The killing effect increased with PAbN concentration and at 100 lg/mL reached 5.1 logs. Conclusions: The EPI PAbN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.",

keywords = "Pseudomonas aeruginosa, antimicrobial photodynamic inactivation, efflux pump inhibitor, methylene blue, phenylalanine-arginine-b-naphthylamide, resistance-nodulation-division family efflux pump",

author = "Lixia He and Shanlin Yang and Weijun Xuan and Xiumei Zhen and Qi Qi and Yongqi Qi and Qingxiang Li and Meixia Du and Hamblin, {Michael R.} and Liyi Huang",

note = "Publisher Copyright: {\textcopyright} Mary Ann Liebert, Inc.",

year = "2023",

month = oct,

day = "1",

doi = "10.1089/photob.2023.0005",

language = "English",

volume = "41",

pages = "569--575",

journal = "Photobiomodulation, Photomedicine, and Laser Surgery",

issn = "2578-5478",

publisher = "Mary Ann Liebert Inc.",

number = "10",

}

TY - JOUR

T1 - Phenylalanine-Arginine-b-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa

AU - He, Lixia

AU - Yang, Shanlin

AU - Xuan, Weijun

AU - Zhen, Xiumei

AU - Qi, Qi

AU - Qi, Yongqi

AU - Li, Qingxiang

AU - Du, Meixia

AU - Hamblin, Michael R.

AU - Huang, Liyi

N1 - Publisher Copyright: © Mary Ann Liebert, Inc.

PY - 2023/10/1

Y1 - 2023/10/1

N2 - Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-b-naphthylamide (PAbN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5–240 min), and then, 10 J/cm2 red light was irradiated. The efflux pump inhibitor (EPI) PAbN (10–100 lg/mL) was combined with MB (1–20 lM) in different sequences (PAbN-first, PAbN+MB, PAbN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 lM plus 10 J/cm2, the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAbN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAbN-first had the best killing effect, whereas PAbN-after had the worst killing effect. The killing effect increased with PAbN concentration and at 100 lg/mL reached 5.1 logs. Conclusions: The EPI PAbN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.

AB - Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-b-naphthylamide (PAbN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5–240 min), and then, 10 J/cm2 red light was irradiated. The efflux pump inhibitor (EPI) PAbN (10–100 lg/mL) was combined with MB (1–20 lM) in different sequences (PAbN-first, PAbN+MB, PAbN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 lM plus 10 J/cm2, the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAbN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAbN-first had the best killing effect, whereas PAbN-after had the worst killing effect. The killing effect increased with PAbN concentration and at 100 lg/mL reached 5.1 logs. Conclusions: The EPI PAbN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.

KW - Pseudomonas aeruginosa

KW - antimicrobial photodynamic inactivation

KW - efflux pump inhibitor

KW - methylene blue

KW - phenylalanine-arginine-b-naphthylamide

KW - resistance-nodulation-division family efflux pump

UR - http://www.scopus.com/inward/record.url?scp=85175587157&partnerID=8YFLogxK

U2 - 10.1089/photob.2023.0005

DO - 10.1089/photob.2023.0005

M3 - Article

C2 - 37870413

AN - SCOPUS:85175587157

SN - 2578-5478

VL - 41

SP - 569

EP - 575

JO - Photobiomodulation, Photomedicine, and Laser Surgery

JF - Photobiomodulation, Photomedicine, and Laser Surgery

IS - 10

ER -

Phenylalanine-Arginine-b-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this