Overoxidized polypyrrole incorporated with gold nanoparticles as platform for impedimetric anti-transglutaminase immunosensor

Natasha West; Priscilla G.L. Baker; Omotayo A. Arotiba; Nicolette R. Hendricks; Abd A. Baleg; Tesfaye T. Waryo; Rachel F. Ngece; Emmanuel I. Iwuoha; C. O'Sullivan

doi:10.1080/00032719.2010.539739

Overoxidized polypyrrole incorporated with gold nanoparticles as platform for impedimetric anti-transglutaminase immunosensor

Natasha West, Priscilla G.L. Baker, Omotayo A. Arotiba, Nicolette R. Hendricks, Abd A. Baleg, Tesfaye T. Waryo, Rachel F. Ngece, Emmanuel I. Iwuoha, C. O'Sullivan

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Overoxidized polypyrrole (OvoxPpy) doped with gold-nanoparticles (GNP) on a glassy carbon electrode (GCE) was used as a platform to construct a novel label-free impedimetric immunosensor for anti-transglutaminase (anti-tTG). Field emission scanning electron microscopy results confirmed the deposition of GNP with good surface coverage across the OvoxPpy substrate. The average diameter of the nanocomposite was 100 nm. The platform (GNP\OvoxPpy\\GCE) was conductive and exhibited reversible electrochemistry (E°' = 395mV) in (pH 7.4) PBS. The electrochemical characterization of the platform was studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). OvoxPpy\ CE had a peak separation, δE _p, value of -80mV. The GNP\ OvoxPpy\ GCE \GCE showed enhanced interfacial charge transfer with δE _p, being -30mV. The SWV results corroborated CV findings which show enhanced peak current through GNP. The immunosensor was prepared by immobilizing 40 μL tTG (0.3mgmL ^-1) onto the platform by drop coating. Electrochemical impedance spectroscopy (EIS) measurements indicated that the immunosensor system (BSA\tTG\GNP\OvoxPpy\\GCE) markedly improved the conductivity and response of the anti-tTG immunosensor. The impedimetric immunosensor exhibited a charge transfer resistance (R _ct)-dependent dynamic linearity of10 ^-6 to 10 ^-4 M for target anti-tTG (r = 0.9808), and detection limit of 5.22 x 10 ^-6 M.

Original language	English
Pages (from-to)	1956-1966
Number of pages	11
Journal	Analytical Letters
Volume	44
Issue number	11
DOIs	https://doi.org/10.1080/00032719.2010.539739
Publication status	Published - Jul 2011
Externally published	Yes

Keywords

Anti-transglutaminase antibodies (ANTI-TTG)
Celiac disease (CD)
Charge transfer resistance (R )
Cyclic voltammetry (CV)
Electrochemical impedance spectroscopy (EIS)
Immunosensor
Square wave voltammetry (SWV)
Transglutaminase (TTG)

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry (medical)
Electrochemistry

Access to Document

10.1080/00032719.2010.539739

Cite this

@article{9d1bb2ebaf0941c9b9ba820974c12aaa,

title = "Overoxidized polypyrrole incorporated with gold nanoparticles as platform for impedimetric anti-transglutaminase immunosensor",

abstract = "Overoxidized polypyrrole (OvoxPpy) doped with gold-nanoparticles (GNP) on a glassy carbon electrode (GCE) was used as a platform to construct a novel label-free impedimetric immunosensor for anti-transglutaminase (anti-tTG). Field emission scanning electron microscopy results confirmed the deposition of GNP with good surface coverage across the OvoxPpy substrate. The average diameter of the nanocomposite was 100 nm. The platform (GNP\OvoxPpy\\GCE) was conductive and exhibited reversible electrochemistry (E°' = 395mV) in (pH 7.4) PBS. The electrochemical characterization of the platform was studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). OvoxPpy\ CE had a peak separation, δE p, value of -80mV. The GNP\ OvoxPpy\ GCE \GCE showed enhanced interfacial charge transfer with δE p, being -30mV. The SWV results corroborated CV findings which show enhanced peak current through GNP. The immunosensor was prepared by immobilizing 40 μL tTG (0.3mgmL -1) onto the platform by drop coating. Electrochemical impedance spectroscopy (EIS) measurements indicated that the immunosensor system (BSA\tTG\GNP\OvoxPpy\\GCE) markedly improved the conductivity and response of the anti-tTG immunosensor. The impedimetric immunosensor exhibited a charge transfer resistance (R ct)-dependent dynamic linearity of10 -6 to 10 -4 M for target anti-tTG (r = 0.9808), and detection limit of 5.22 x 10 -6 M.",

keywords = "Anti-transglutaminase antibodies (ANTI-TTG), Celiac disease (CD), Charge transfer resistance (R ), Cyclic voltammetry (CV), Electrochemical impedance spectroscopy (EIS), Immunosensor, Square wave voltammetry (SWV), Transglutaminase (TTG)",

author = "Natasha West and Baker, {Priscilla G.L.} and Arotiba, {Omotayo A.} and Hendricks, {Nicolette R.} and Baleg, {Abd A.} and Waryo, {Tesfaye T.} and Ngece, {Rachel F.} and Iwuoha, {Emmanuel I.} and C. O'Sullivan",

year = "2011",

month = jul,

doi = "10.1080/00032719.2010.539739",

language = "English",

volume = "44",

pages = "1956--1966",

journal = "Analytical Letters",

issn = "0003-2719",

publisher = "Taylor and Francis Ltd.",

number = "11",

}

TY - JOUR

T1 - Overoxidized polypyrrole incorporated with gold nanoparticles as platform for impedimetric anti-transglutaminase immunosensor

AU - West, Natasha

AU - Baker, Priscilla G.L.

AU - Arotiba, Omotayo A.

AU - Hendricks, Nicolette R.

AU - Baleg, Abd A.

AU - Waryo, Tesfaye T.

AU - Ngece, Rachel F.

AU - Iwuoha, Emmanuel I.

AU - O'Sullivan, C.

PY - 2011/7

Y1 - 2011/7

N2 - Overoxidized polypyrrole (OvoxPpy) doped with gold-nanoparticles (GNP) on a glassy carbon electrode (GCE) was used as a platform to construct a novel label-free impedimetric immunosensor for anti-transglutaminase (anti-tTG). Field emission scanning electron microscopy results confirmed the deposition of GNP with good surface coverage across the OvoxPpy substrate. The average diameter of the nanocomposite was 100 nm. The platform (GNP\OvoxPpy\\GCE) was conductive and exhibited reversible electrochemistry (E°' = 395mV) in (pH 7.4) PBS. The electrochemical characterization of the platform was studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). OvoxPpy\ CE had a peak separation, δE p, value of -80mV. The GNP\ OvoxPpy\ GCE \GCE showed enhanced interfacial charge transfer with δE p, being -30mV. The SWV results corroborated CV findings which show enhanced peak current through GNP. The immunosensor was prepared by immobilizing 40 μL tTG (0.3mgmL -1) onto the platform by drop coating. Electrochemical impedance spectroscopy (EIS) measurements indicated that the immunosensor system (BSA\tTG\GNP\OvoxPpy\\GCE) markedly improved the conductivity and response of the anti-tTG immunosensor. The impedimetric immunosensor exhibited a charge transfer resistance (R ct)-dependent dynamic linearity of10 -6 to 10 -4 M for target anti-tTG (r = 0.9808), and detection limit of 5.22 x 10 -6 M.

AB - Overoxidized polypyrrole (OvoxPpy) doped with gold-nanoparticles (GNP) on a glassy carbon electrode (GCE) was used as a platform to construct a novel label-free impedimetric immunosensor for anti-transglutaminase (anti-tTG). Field emission scanning electron microscopy results confirmed the deposition of GNP with good surface coverage across the OvoxPpy substrate. The average diameter of the nanocomposite was 100 nm. The platform (GNP\OvoxPpy\\GCE) was conductive and exhibited reversible electrochemistry (E°' = 395mV) in (pH 7.4) PBS. The electrochemical characterization of the platform was studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). OvoxPpy\ CE had a peak separation, δE p, value of -80mV. The GNP\ OvoxPpy\ GCE \GCE showed enhanced interfacial charge transfer with δE p, being -30mV. The SWV results corroborated CV findings which show enhanced peak current through GNP. The immunosensor was prepared by immobilizing 40 μL tTG (0.3mgmL -1) onto the platform by drop coating. Electrochemical impedance spectroscopy (EIS) measurements indicated that the immunosensor system (BSA\tTG\GNP\OvoxPpy\\GCE) markedly improved the conductivity and response of the anti-tTG immunosensor. The impedimetric immunosensor exhibited a charge transfer resistance (R ct)-dependent dynamic linearity of10 -6 to 10 -4 M for target anti-tTG (r = 0.9808), and detection limit of 5.22 x 10 -6 M.

KW - Anti-transglutaminase antibodies (ANTI-TTG)

KW - Celiac disease (CD)

KW - Charge transfer resistance (R )

KW - Cyclic voltammetry (CV)

KW - Electrochemical impedance spectroscopy (EIS)

KW - Immunosensor

KW - Square wave voltammetry (SWV)

KW - Transglutaminase (TTG)

UR - http://www.scopus.com/inward/record.url?scp=80051689195&partnerID=8YFLogxK

U2 - 10.1080/00032719.2010.539739

DO - 10.1080/00032719.2010.539739

M3 - Article

AN - SCOPUS:80051689195

SN - 0003-2719

VL - 44

SP - 1956

EP - 1966

JO - Analytical Letters

JF - Analytical Letters

IS - 11

ER -

Overoxidized polypyrrole incorporated with gold nanoparticles as platform for impedimetric anti-transglutaminase immunosensor

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this