TY - JOUR
T1 - One-Step Synthesis of Metal Oxide Nanoparticles Using Cannabidiol
T2 - Characterisation and Cytotoxicity Assessment in Human Keratinocyte Cells
AU - Josiah, Andrea Jess
AU - Pillai, Sreejarani Kesavan
AU - Cordier, Werner
AU - Nell, Margo
AU - Lall, Namrita
AU - Twilley, Danielle
AU - Ray, Suprakas Sinha
N1 - Publisher Copyright:
© 2024 The Authors. ChemistrySelect published by Wiley-VCH GmbH.
PY - 2024/2/26
Y1 - 2024/2/26
N2 - This research paper explores the field of nanobiotechnology, focusing on the design, characterisation, and potential dermal applications of metal oxide nanoparticles (MONPs). ZnO and FeO NPs exhibit distinctive properties that are valuable in dermato-cosmetic applications and transdermal drug delivery. This study investigates Cannabidiol (CBD) as a capping agent for MONPs synthesis. Employing microwave-assisted techniques, MONPs were synthesised using either CBD or polyvinylpyrrolidone (PVP) as capping agents. The TEM, SEM, FTIR, and XRD characterisation results confirmed the successful formation of CBD-capped ZnO and FeO NPs exhibiting an average particle size of 90 and 76 nm, respectively. The cytotoxicity of CBD-capped MONPs was evaluated on HaCaT cells over a concentration range of 100 to 6.25 μg/mL, which revealed that CBD-capped ZnO NPs exerted a cytotoxic effect on HaCaT cells (IC50 85.34±1.17 μg/mL). In contrast, CBD-capped FeO NPs and PVP-capped MONPs exhibited negligible cytotoxicity (IC50 >100 μg/mL). TEM analysis revealed a noticeable structural alteration of ZnO NPs in the supplemented cell culture medium, which could contribute to enhanced NP uptake, thereby explaining the more pronounced cytotoxic effect of ZnO NPs. Therefore, the disparity in cytotoxic responses can be attributed to the protein coating adhering to the NPs surface in a biological medium.
AB - This research paper explores the field of nanobiotechnology, focusing on the design, characterisation, and potential dermal applications of metal oxide nanoparticles (MONPs). ZnO and FeO NPs exhibit distinctive properties that are valuable in dermato-cosmetic applications and transdermal drug delivery. This study investigates Cannabidiol (CBD) as a capping agent for MONPs synthesis. Employing microwave-assisted techniques, MONPs were synthesised using either CBD or polyvinylpyrrolidone (PVP) as capping agents. The TEM, SEM, FTIR, and XRD characterisation results confirmed the successful formation of CBD-capped ZnO and FeO NPs exhibiting an average particle size of 90 and 76 nm, respectively. The cytotoxicity of CBD-capped MONPs was evaluated on HaCaT cells over a concentration range of 100 to 6.25 μg/mL, which revealed that CBD-capped ZnO NPs exerted a cytotoxic effect on HaCaT cells (IC50 85.34±1.17 μg/mL). In contrast, CBD-capped FeO NPs and PVP-capped MONPs exhibited negligible cytotoxicity (IC50 >100 μg/mL). TEM analysis revealed a noticeable structural alteration of ZnO NPs in the supplemented cell culture medium, which could contribute to enhanced NP uptake, thereby explaining the more pronounced cytotoxic effect of ZnO NPs. Therefore, the disparity in cytotoxic responses can be attributed to the protein coating adhering to the NPs surface in a biological medium.
KW - Cannabidiol
KW - Cytotoxicity
KW - Material Characterisation
KW - Metal Oxide Nanoparticles
KW - Microwave-Assisted Synthesis
UR - http://www.scopus.com/inward/record.url?scp=85185685061&partnerID=8YFLogxK
U2 - 10.1002/slct.202304373
DO - 10.1002/slct.202304373
M3 - Article
AN - SCOPUS:85185685061
SN - 2365-6549
VL - 9
JO - ChemistrySelect
JF - ChemistrySelect
IS - 8
M1 - e202304373
ER -