TY - JOUR
T1 - Occurrence of cucumber mosaic virus Subgroup I on different plant families in Himachal Pradesh and its infection to new hosts
AU - Kumar, Ashwini
AU - Jain, Rakesh Kumar
AU - Bhattarai, Ajay
AU - Rathore, Aayushi Singh
AU - Watpade, Santosh G.
AU - Parkash, Chander
AU - Baranwal, Virendra Kumar
AU - Pant, Rajendra Prasad
AU - Kaur, Baljeet
AU - Basavaraj, Y. B.
N1 - Publisher Copyright:
© 2020, Indian Phytopathological Society.
PY - 2020/12
Y1 - 2020/12
N2 - Cucumber mosaic virus (CMV) has been recorded worldwide, infecting more than 1200 plant species including weeds. In this study, 23 samples of bell pepper, squash, tomato, ornamental (calla lily, French hydrangea, spider lily) and weeds (Arrowleaf dock, Spanish needle) hosts originating from Himachal Pradesh were examined. 20 of 23 samples were identified CMV positive based on electron microscopy, bio-assay, RT-PCR assay and sequence analyses of coat protein (CP) gene. The CMV-CP gene sequences from eight isolates cloned and sequenced in this study shared 96–100% amino acid sequence identities with CMV Subgroup I isolates. This study forms the first report of the natural occurrence of CMV on squash and a weed host, Arrowleaf dock (Rumex hastatus), in India as well as the occurrence of CMV Subgroup I in the cooler hilly regions of India. These findings suggest the expansion of the host range as well as ecological niche of CMV.
AB - Cucumber mosaic virus (CMV) has been recorded worldwide, infecting more than 1200 plant species including weeds. In this study, 23 samples of bell pepper, squash, tomato, ornamental (calla lily, French hydrangea, spider lily) and weeds (Arrowleaf dock, Spanish needle) hosts originating from Himachal Pradesh were examined. 20 of 23 samples were identified CMV positive based on electron microscopy, bio-assay, RT-PCR assay and sequence analyses of coat protein (CP) gene. The CMV-CP gene sequences from eight isolates cloned and sequenced in this study shared 96–100% amino acid sequence identities with CMV Subgroup I isolates. This study forms the first report of the natural occurrence of CMV on squash and a weed host, Arrowleaf dock (Rumex hastatus), in India as well as the occurrence of CMV Subgroup I in the cooler hilly regions of India. These findings suggest the expansion of the host range as well as ecological niche of CMV.
KW - Arrowleaf dock (Rumex hastatus)
KW - Coat protein
KW - Cucumber mosaic virus
KW - Squash
KW - Subgroup I
UR - http://www.scopus.com/inward/record.url?scp=85087764421&partnerID=8YFLogxK
U2 - 10.1007/s42360-020-00263-6
DO - 10.1007/s42360-020-00263-6
M3 - Article
AN - SCOPUS:85087764421
SN - 0367-973X
VL - 73
SP - 759
EP - 766
JO - Indian Phytopathology
JF - Indian Phytopathology
IS - 4
ER -
