Multimodal quantitative imaging of brain cancer in cultured cells

X. I.N. Feng; Alona Muzikansky; Alonzo H. Ross; Michael R. Hamblin; Peter R. Jermain; Anna N. Yaroslavsky

doi:10.1364/BOE.10.004237

Multimodal quantitative imaging of brain cancer in cultured cells

X. I.N. Feng
, Alona Muzikansky
, Alonzo H. Ross
, Michael R. Hamblin
, Peter R. Jermain
, Anna N. Yaroslavsky

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

Original language	English
Article number	364428
Pages (from-to)	4237-4248
Number of pages	12
Journal	Biomedical Optics Express
Volume	10
Issue number	8
DOIs	https://doi.org/10.1364/BOE.10.004237
Publication status	Published - 1 Aug 2019
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.10.004237

Cite this

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title = "Multimodal quantitative imaging of brain cancer in cultured cells",

abstract = "Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.",

author = "Feng, \{X. I.N.\} and Alona Muzikansky and Ross, \{Alonzo H.\} and Hamblin, \{Michael R.\} and Jermain, \{Peter R.\} and Yaroslavsky, \{Anna N.\}",

note = "Publisher Copyright: {\textcopyright} 2019 Optical Society of America.",

year = "2019",

month = aug,

day = "1",

doi = "10.1364/BOE.10.004237",

language = "English",

volume = "10",

pages = "4237--4248",

journal = "Biomedical Optics Express",

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publisher = "Optica Publishing Group (formerly OSA)",

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TY - JOUR

T1 - Multimodal quantitative imaging of brain cancer in cultured cells

AU - Feng, X. I.N.

AU - Muzikansky, Alona

AU - Ross, Alonzo H.

AU - Hamblin, Michael R.

AU - Jermain, Peter R.

AU - Yaroslavsky, Anna N.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

AB - Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

UR - https://www.scopus.com/pages/publications/85070974639

U2 - 10.1364/BOE.10.004237

DO - 10.1364/BOE.10.004237

M3 - Article

AN - SCOPUS:85070974639

SN - 2156-7085

VL - 10

SP - 4237

EP - 4248

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 8

M1 - 364428

ER -

Multimodal quantitative imaging of brain cancer in cultured cells

Abstract

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ASJC Scopus subject areas

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