Multimodal quantitative imaging of brain cancer in cultured cells

X. I.N. Feng; Alona Muzikansky; Alonzo H. Ross; Michael R. Hamblin; Peter R. Jermain; Anna N. Yaroslavsky

doi:10.1364/BOE.10.004237

Multimodal quantitative imaging of brain cancer in cultured cells

X. I.N. Feng, Alona Muzikansky, Alonzo H. Ross, Michael R. Hamblin, Peter R. Jermain, Anna N. Yaroslavsky

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

Original language	English
Article number	364428
Pages (from-to)	4237-4248
Number of pages	12
Journal	Biomedical Optics Express
Volume	10
Issue number	8
DOIs	https://doi.org/10.1364/BOE.10.004237
Publication status	Published - 1 Aug 2019
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.10.004237

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title = "Multimodal quantitative imaging of brain cancer in cultured cells",

abstract = "Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.",

author = "Feng, {X. I.N.} and Alona Muzikansky and Ross, {Alonzo H.} and Hamblin, {Michael R.} and Jermain, {Peter R.} and Yaroslavsky, {Anna N.}",

note = "Publisher Copyright: {\textcopyright} 2019 Optical Society of America.",

year = "2019",

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doi = "10.1364/BOE.10.004237",

language = "English",

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T1 - Multimodal quantitative imaging of brain cancer in cultured cells

AU - Feng, X. I.N.

AU - Muzikansky, Alona

AU - Ross, Alonzo H.

AU - Hamblin, Michael R.

AU - Jermain, Peter R.

AU - Yaroslavsky, Anna N.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

AB - Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 8

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ER -

Multimodal quantitative imaging of brain cancer in cultured cells

Abstract

ASJC Scopus subject areas

UN SDGs

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